FlyBase Allele Report: Dmel\nolo[Δ4-12]

General Information

Symbol

Dmel\nolo^Δ4-12

Species

D. melanogaster

Name

FlyBase ID

FBal0299636

Feature type

allele

Associated gene

Dmel\nolo

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Meyer et al., 2014)

Mutagen

FLPase

(Meyer et al., 2014)

Progenitor genotype

PBac{RB}nolo^e02659

(Meyer et al., 2014)

PBac{WH}nolo^f05136

(Meyer et al., 2014)

Cytology

Description

Recombination between the progenitor insertions removes exons 4-12 and a large part of the open reading frame of nolo.

(Meyer et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:21,690,131..21,713,608 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them.

(Meyer et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | larval stage

(Meyer et al., 2014)

abnormal locomotor behavior | larval stage

(Meyer et al., 2014)

flightless

(Meyer et al., 2014)

partially lethal - majority die | maternal effect

(Meyer et al., 2014)

viable

(Meyer et al., 2014)

Phenotype Manifest In

wing

(Meyer et al., 2014)

Detailed Description

Statement

Reference

Mutants are viable but flightless and extend their wings abnormally. Maternally and zygotic mutant animals are also viable but survival rates are reduced. The length of the ventral nerve cord is not extended in zygotic or maternal/zygotic mutants.

Mutants show abnormal larval migratory behaviour, crawl slower than wild type, and do not recover and walk properly in a 'RING assay'. Head sweeping behaviour is abnormal.

(Meyer et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

nolo^Δ4-12 has abnormal behavior phenotype, enhanceable by Oatp30B^Δ2-14

(Meyer et al., 2014)

Other

Statement

Reference

Oatp30B^Δ2-14, nolo^Δ4-12 has partially lethal - majority die phenotype

(Meyer et al., 2014)

Oatp30B^Δ2-14, nolo^Δ4-12 has female sterile phenotype

(Meyer et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

In contrast to either single mutant, nolo^Δ4-12 Oatp30B^Δ2-14 double mutants show dramatically reduced rates of eclosion and female sterility. The ventral nerve cord is shaped normally in double mutants. Head sweeping behaviour in double mutant larvae is dramatically reduced compared to either single mutant.

(Meyer et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

nolo^Δ4-12 is rescued by nolo^UAS.EGFP/Scer\GAL4^repo.PU

(Meyer et al., 2014)

Partially rescued by

nolo^Δ4-12 is partially rescued by nolo^UAS.EGFP/Scer\GAL4^da.G32

(Meyer et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DF(2L)nolo^Δ4-12

(Meyer et al., 2014)

nolo^Δ4-12

noloΔ^4-12

(Meyer et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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