FlyBase Allele Report: Dmel\mir-277[sponge.UASp.EGFP]

General Information

Symbol

Dmel\mir-277^{sponge.UASp.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0299693

Feature type

allele

Associated gene

Dmel\mir-277

Associated Insertion(s)

Carried in Construct

P{UASp-eGFP.mir-277.sponge}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Tan et al., 2012)

Progenitor genotype

Carried in construct

P{UASp-eGFP.mir-277.sponge}

Cytology

Description

UASp regulatory sequences drive expression of a mir-276a "sponge" sequence, which consists of ten repetitive mir-276a complementary sequences with mismatches at positions 9-12 separated by a variable four-nucleotide linker. The sponge sequence is inserted into 3'UTR sequence downstream of the EGFP coding region.

(Tan et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

mir-277

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of mir-277^{sponge.Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF has no effect on eye morphology.

Expression of Avic\GFP^{EGFP.Scer\UAS.P\T.mir-276aSP} under the control of Scer\GAL4^GMR.PF has no effect on eye morphology.

(Tan et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mir-277^{sponge.UASp.EGFP}/Scer\GAL4^GMR.PF is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Tan et al., 2012)

mir-277^{sponge.UASp.EGFP}/Scer\GAL4^GMR.PF is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Tan et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

mir-277^{sponge.UASp.EGFP}/Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Tan et al., 2012)

mir-277^{sponge.UASp.EGFP}/Scer\GAL4^GMR.PF is a suppressor | partially of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CGG^90.UAS.EGFP

(Tan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Avic\GFP^{EGFP.Scer\UAS.P\T.mir-276aSP} partially suppresses the fused ommatidia phenotype seen when Avic\GFP^{Fmr1.CGG90.E.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF.

Expression of mir-277^{sponge.Scer\UAS.P\T.T:Avic\GFP-EGFP} partially suppresses the fused ommatidia phenotype seen when Zzzz\CGG^{90.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^GMR.PF.

(Tan et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Avic\GFP^{EGFP.Scer\UAS.P\T.mir-276aSP}

mir-277^{sponge.Scer\UAS.P\T.T:Avic\GFP-EGFP}

mir-277^{sponge.UASp.EGFP}

Name Synonyms

Secondary FlyBase IDs

FBal0280059

References (2)

Report Sections

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