FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\spn-F1-280.UASp.N.GFP
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General Information
Symbol
Dmel\spn-F1-280.UASp.N.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0300985
Feature type
allele
Associated gene
Dmel\spn-F
Associated Insertion(s)
Carried in Construct
P{UASp-GFP-spn-F.1-280}
Key Links
Transgenic product class
inframe_deletion
(Amsalem et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Amsalem et al., 2013)
Mutagen
in vitro construct
(Amsalem et al., 2013)
Progenitor genotype
Carried in construct
P{UASp-GFP-spn-F.1-280}
Cytology
Description

UASp regulatory sequences drive expression of truncated spn-F sequences corresponding to residues 1-280 of spn-F, containing two coiled-coil domains but lacking the 84 C-terminal residues. Tagged at the N-terminal with GFP.

(Amsalem et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
spn-F
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to the appearance of ventralized eggshell and bicaudal embryos.

      8% of eggshells from spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP-expressing flies exhibit a wild-type appearance, while 13% display a weakly ventralized eggshell with fused dorsal appendages, and 76% show a ventralized eggshell with one dorsal appendage and the remaining 3% show

      a strongly ventralized eggshell with no dorsal appendages.

      Approximately 98% of embryos from flies expressing spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 exhibit a bicaudal phenotype.

      Expression of spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 leads to complete female sterility.

      Approximately 92% of eggs laid by female flies expressing spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP in the germline produce ventralized embryos.

      Expression of spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP in the germline (under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16) severely affects the embryonic anterior-posterior axis.

      Approximately 98% of the embryos produced by females expressing spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP in the germline (under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16) show a strong bicaudal phenotype, with several abdominal segments appearing in mirror image symmetry, and Filzkoerper and telson at both ends.

      Expression of spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP in the germline, under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16, affects oocyte actin organisation.

      GA28a. Genetic interaction (class, effect) [CV] *S :embryonic/larval segmentation phenotype | maternal effect { Scer\GAL4[mat.αTub67C. T:Hsim \VP16] }, suppressible { ik2[Scer\UAS.P\T. T:Avic \GFP-EGFP], Scer\GAL4[mat.αTub67C. T:Hsim \VP16] }

      (Amsalem et al., 2013)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of ik2Scer\UAS.P\T.T:Avic\GFP-EGFP significantly suppresses spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP-induced ovarian phenotypes.

      Co-expression of ik2Scer\UAS.P\T.T:Avic\GFP-EGFP suppresses spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP-induced eggshell ventralization phenotype.

      Co-expression of ik2Scer\UAS.P\T.T:Avic\GFP-EGFP reduces the spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP-induced bicaudal embryo phenotype from 98% to 39%.

      (Amsalem et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4VP16.mat.αTub67C/spn-F1-280.UASp.N.GFP fails to rescue spn-F1

      (Amsalem et al., 2013)
      Comments

      Expression of spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 fails to rescue spn-F1 female sterility.

      (Amsalem et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      spn-F1-280.Scer\UAS.P\T.N.T:Avic\GFP
      spn-F1-280.UASp.N.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)