Flies expressing Acnt.D527A (from the endogenous promoter) exhibit increased autophagy.
Atg8a-positive autophagosomes form at a rate significantly higher in Acnt.D527A expressing eye discs than in controls, indicating increased autophagy.
There is an elevated level of starvation-independent basal autophagy at all stages when Acn cleavage at asp is blocked (as is the case in Acnt.D527A mutants).
Flies carrying one copy of Acnt.D527A survive significantly longer in the absence of food than wild-type flies.
Flies carrying one copy of Acnt.D527A exhibit greater longevity than control flies, with the median life span extended by 50%.