FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\AcnD527A.Tag:MYC
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General Information
Symbol
Dmel\AcnD527A.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0301142
Feature type
allele
Associated gene
Dmel\Acn
Associated Insertion(s)
Carried in Construct
PBac{AcnP-Myc-Acn.D527A}
Key Links
Transgenic product class
missense_variant
(Nandi et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Nandi et al., 2014)
Mutagen
in vitro construct
(Nandi et al., 2014)
Progenitor genotype
Carried in construct
PBac{AcnP-Myc-Acn.D527A}
Cytology
Description

4kb genomic fragment that encompasses the Acn gene. The coding sequence has been mutated to carry the D527A amino acid replacement (mutation is within the caspase cleavage site) and is tagged at the N-terminal end with Tag:MYC.

(Nandi et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
Acn
Encoded product / tool
Acn
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing Acnt.D527A (from the endogenous promoter) exhibit increased autophagy.

      Atg8a-positive autophagosomes form at a rate significantly higher in Acnt.D527A expressing eye discs than in controls, indicating increased autophagy.

      There is an elevated level of starvation-independent basal autophagy at all stages when Acn cleavage at asp[527] is blocked (as is the case in Acnt.D527A mutants).

      Flies carrying one copy of Acnt.D527A survive significantly longer in the absence of food than wild-type flies.

      Flies carrying one copy of Acnt.D527A exhibit greater longevity than control flies, with the median life span extended by 50%.

      (Nandi et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhancer of
      Statement
      Reference

      AcnD527A.Tag:MYC is a non-enhancer of photoreceptor neuron phenotype of Hsap\HTTQ93.ex1.UAS, Scer\GAL4GMR.PF

      (Nandi et al., 2014)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      In two week-old flies, one copy of Acnt.D527A significantly improves the neuronal depolarization seen in Hsap\HTTQ93.ex1p.Scer\UAS-expressing flies.

      The build up of aggregates seen in two week-old Hsap\HTTQ93.ex1p.Scer\UAS-expressing flies is reduced by the presence of one copy of Acnt.D527A.

      One copy of Acnt.D527A does not affect the neuronal depolarization seen in 2 week old Hsap\HTTQ93.ex1p.Scer\UAS-expressing flies.

      (Nandi et al., 2014)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      AcnD527A.Tag:MYC
      Acnt.D527A
      Name Synonyms
      Secondary FlyBase IDs
        References (2)