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General Information
Symbol
Dmel\AcnD527A.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0301142
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

4kb genomic fragment that encompasses the Acn gene. The coding sequence has been mutated to carry the D527A amino acid replacement (mutation is within the caspase cleavage site) and is tagged at the N-terminal end with Tag:MYC.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Acnt.D527A (from the endogenous promoter) exhibit increased autophagy.

Atg8a-positive autophagosomes form at a rate significantly higher in Acnt.D527A expressing eye discs than in controls, indicating increased autophagy.

There is an elevated level of starvation-independent basal autophagy at all stages when Acn cleavage at asp[527] is blocked (as is the case in Acnt.D527A mutants).

Flies carrying one copy of Acnt.D527A survive significantly longer in the absence of food than wild-type flies.

Flies carrying one copy of Acnt.D527A exhibit greater longevity than control flies, with the median life span extended by 50%.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhancer of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

In two week-old flies, one copy of Acnt.D527A significantly improves the neuronal depolarization seen in Hsap\HTTQ93.ex1p.Scer\UAS-expressing flies.

The build up of aggregates seen in two week-old Hsap\HTTQ93.ex1p.Scer\UAS-expressing flies is reduced by the presence of one copy of Acnt.D527A.

One copy of Acnt.D527A does not affect the neuronal depolarization seen in 2 week old Hsap\HTTQ93.ex1p.Scer\UAS-expressing flies.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
AcnD527A.Tag:MYC
Acnt.D527A
Name Synonyms
Secondary FlyBase IDs
    References (2)