FlyBase Allele Report: Dmel\CycB[5A.UASp.GFP]

General Information

Symbol

Dmel\CycB^5A.UASp.GFP

Species

D. melanogaster

Name

FlyBase ID

FBal0301298

Feature type

allele

Associated gene

Dmel\CycB

Associated Insertion(s)

Carried in Construct

P{UASp-CycB-GFP.5A}

Key Links

Transgenic product class

missense_variant

(Mathieu et al., 2013)

polypeptide_post_translational_processing_variant

(Mathieu et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Mathieu et al., 2013)

polypeptide_post_translational_processing_variant

(Mathieu et al., 2013)

Mutagen

in vitro construct

(Mathieu et al., 2013)

Progenitor genotype

Carried in construct

P{UASp-CycB-GFP.5A}

Cytology

Description

Amino acid replacement: T27A.

(Mathieu et al., 2013)

UASp regulatory sequences drive expression of full-length mutated CycB tagged at the C-terminal with GFP. Containing 5 amino acid replacements that remove aurB phosphorylation sites.

(Mathieu et al., 2013)

Amino acid replacement: T125A.

(Mathieu et al., 2013)

Amino acid replacement: T132A.

(Mathieu et al., 2013)

Amino acid replacement: T157A.

(Mathieu et al., 2013)

Amino acid replacement: T200A.

(Mathieu et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

CycB

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell cycle, with Scer\GAL4^VP16.bam

(Mathieu et al., 2013)

Phenotype Manifest In

egg chamber, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female fusome, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female germline cyst, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

female germline stem cell, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

ring canal, with Scer\GAL4^{VP16.nanos.UTR}

(Mathieu et al., 2013)

Detailed Description

Statement

Reference

Expression of CycB^{5A.Scer\UAS.P\T.T:Avic\GFP} in germline cysts, under the control of Scer\GAL4^{bam.T:Hsim\VP16} induces a significant number of 32-cell cysts, while expression in all germline cells of the germarium, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not affect germline cyst division.

Expression of CycB^{5A.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} induces the appearance of breaking fusome in two-cell cysts. Abscission appears to be premature in germline stem cells and two-cell cysts.

Expression of CycB^{5A.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{bam.T:Hsim\VP16} does not generate a phenotype.

(Mathieu et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP is a suppressor of egg chamber phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP is a suppressor of germline cell phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP is a suppressor of ring canal phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP is a suppressor of nurse cell phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP is a suppressor of female germline cyst phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

Scer\GAL4^VP16.bam/CycB^5A.UASp.GFP is a suppressor of female fusome phenotype of aurB¹⁶⁸⁹

(Mathieu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of CycB^{5A.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{bam.T:Hsim\VP16} in aurB¹⁶⁸⁹ mutant cysts induces a strong reduction in the number of eight-cell cysts from 14.5% to 3.5% of eight-cell cysts.

(Mathieu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

CycB^5A.UASp.GFP rescues CycB³/CycB¹

(Mathieu et al., 2013)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CycB^{5A.Scer\UAS.P\T.T:Avic\GFP}

CycB^5A.UASp.GFP

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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