FlyBase Allele Report: Dmel\β-PheRS[+tLa]

General Information

Symbol

Dmel\β-PheRS^+tLa

Species

D. melanogaster

Name

Genomic construct a of Lu

FlyBase ID

FBal0301680

Feature type

allele

Associated gene

Dmel\β-PheRS

Associated Insertion(s)

Carried in Construct

M{β-PheRS^+tLa}

Key Links

Genomic Maps

JBrowse

Transgenic product class

genomic_DNA

(Lu et al., 2014)

wild_type

(Lu et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Lu et al., 2014)

wild_type

(Lu et al., 2014)

Mutagen

in vitro construct

(Lu et al., 2014)

Progenitor genotype

Carried in construct

M{β-PheRS^+tLa}

Cytology

Description

A genomic construct containing the entire coding region of β-PheRS and ~1kb of upstream and downstream sequences.

(Lu et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

β-PheRS

Encoded product / tool

β-PheRS

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

rescue_region

3R:24,249,524..24,253,553 [-]

Comment:

Approximate extent of rescue construct that carries the coding region of beta-PheRS plus ~1 kb upstream and downstream.

(Lu et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^Act5C.PI has increased cell death phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has increased cell death phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has decreased cell number phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has abnormal size phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^ppl.PP has lethal | nutrition conditional phenotype

(Lu et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^en-e16E has wing phenotype

(Lu et al., 2014)

α-PheRS^A456G.UAS, β-PheRS^+tLa, Df(3R)Exel6198/β-PheRS³²², Scer\GAL4^ey.PH has ommatidium phenotype

(Lu et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

In β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS^+tLa, Scer\GAL4^ey.PH-mediated expression of α-PheRS^{A456G.Scer\UAS}, results in eyes mostly displaying a regular surface, although a few ommatidia are not properly organized in certain regions. Some individual ones lack photoreceptors, causing ommatidia to be smaller and the retina to display a misaligned pattern.

Scer\GAL4^Act5C.PI-mediated expression of α-PheRS^{A456G.Scer\UAS} in adults (via use of Scer\GAL80^ts.αTub84B) in a β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) background rescued with β-PheRS^+tLa results in some elav-positive brain (neuronal) cells entering apoptosis.

In β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS^+tLa, Scer\GAL4^en-e16E-mediated expression of α-PheRS^{A456G.Scer\UAS} results in decreased wing size , and in some wings entire regions of the posterior compartment are missing. In wing discs, the number of apoptotic cells is increased, while cell size is either similar or slightly bigger than controls, suggesting that cell number (proliferation) must be reduced.

In β-PheRS³²²/Df(3R)Exel6198 (β-PheRS null) flies rescued with β-PheRS^+tLa, Scer\GAL4^ppl.PP-mediated expression of α-PheRS^{A456G.Scer\UAS} results in reduced pupariation rates when larvae are raised on Tyrosine-rich (but not alanine- or phenylalanine-rich or standard) food.

(Lu et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

β-PheRS^+tLa rescues β-PheRS³²²

(Lu et al., 2014)

β-PheRS^+tLa rescues Df(3R)Exel6198/β-PheRS³²²

(Lu et al., 2014)

Comments

β-PheRS^+tLa rescues the lethality of β-PheRS³²² and β-PheRS³²²/Df(3R)Exel6198.