FlyBase Allele Report: Dmel\mir-316[KO]

General Information

Symbol

Dmel\mir-316^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0301921

Feature type

allele

Associated gene

Dmel\mir-316

Associated Insertion(s)

TI{TI}mir-316^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chen et al., 2014)

Mutagen

ends-out gene targeting

(Chen et al., 2014)

FLPase

(Chen et al., 2014)

I-SceI

(Chen et al., 2014)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-316^KO

Cytology

Description

The mir-316 hairpin has been deleted and replaced by a loxP cassette containing the w^+mW.hs marker.

(Chen et al., 2014)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile (with Df(3L)ED4978)

(Chen et al., 2014)

male fertile

(Chen et al., 2014)

male fertile (with Df(3L)ED4978)

(Chen et al., 2014)

male semi-fertile

(Iki et al., 2020)

some die during embryonic stage (with Df(3L)ED4978)

(Chen et al., 2014)

some die during embryonic stage | recessive | maternal effect | recessive

(Chen et al., 2014)

Phenotype Manifest In

germline cell | embryonic stage (with Df(3L)ED4978)

(Chen et al., 2014)

germline cell | embryonic stage | maternal effect (with Df(3L)ED4978)

(Chen et al., 2014)

sperm individualization complex

(Iki et al., 2020)

testis

(Iki et al., 2020)

Detailed Description

Statement

Reference

mir-316^KO testis exhibit defects in the individualization complex organization when compared to controls.

(Iki et al., 2020)

Embryos derived from mir-316^KO/Df(3L)ED4978 embryos show a significant increase in the number of primordial germ cells compared to embryos from control females.

mir-316^KO/Df(3L)ED4978 embryos show a significant increase in primordial germ cell number compared to controls.

Homozygous adults show significant defects in the hemolymph-brain barrier (as determined using a dye injection assay).

(Chen et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mir-316^KO is rescued by Scer\GAL4^bam.PU/mir-316^RNAi.UAS.3p

(Iki et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58925

w^*; TI{TI}mir-316^KO/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

miR-316^KO

(Iki et al., 2023, Iki et al., 2020)

mir-316 null

(McNeill et al., 2020)

mir-316^KO

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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