FlyBase Allele Report: Dmel\ND-42[UAS.cMa]

General Information

Symbol

Dmel\ND-42^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Morais

FlyBase ID

FBal0302207

Feature type

allele

Associated gene

Dmel\ND-42

Associated Insertion(s)

Carried in Construct

PBac{UAS-ND-42.M}

Key Links

Transgenic product class

cDNA

(Morais et al., 2014)

wild_type

(Morais et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Morais et al., 2014)

wild_type

(Morais et al., 2014)

Mutagen

in vitro construct

(Morais et al., 2014)

Progenitor genotype

Carried in construct

PBac{UAS-ND-42.M}

Cytology

Description

UAS regulatory sequences drive expression of a ND-42 cDNA clone (LD29280).

(Morais et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ND-42

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Pogson et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ND-42^UAS.cMa/Scer\GAL4^da.PU is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Pogson et al., 2014)

NOT Suppressor of

Statement

Reference

ND-42^UAS.cMa/Scer\GAL4^da.PU is a non-suppressor of abnormal neurophysiology phenotype of Pink1^B9

(Morais et al., 2014)

ND-42^UAS.cMa/Scer\GAL4^da.PU is a non-suppressor of abnormal flight phenotype of Pink1^B9

(Pogson et al., 2014)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

ND-42^UAS.cMa/Scer\GAL4^da.PU is a non-suppressor of mitochondrion phenotype of Pink1^B9

(Morais et al., 2014)

ND-42^UAS.cMa/Scer\GAL4^da.PU is a non-suppressor of mitochondrial crista phenotype of Pink1^B9

(Morais et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ND-42^Scer\UAS.cMa under the control of Scer\GAL4^da.PU does not rescue the defects in NMJ neurotransmitter release seen in Pink1^B9 mutants. Basal neurotransmitter release is unaffected in these flies. The defect in reserve pool vesicle loading, decrease in mitochondrial membrane potential at NMJ boutons and decreased ATP content are also not rescued and there is no improvement in the structural organisation of the mitochondrial cristae in the flight muscles.

(Morais et al., 2014)

Ubiquitous expression of ND-42^Scer\UAS.cMa under the control of Scer\GAL4^da.PU partially rescues the climbing defects seen in Pink1^B9 mutant flies. The flight defects are not significantly rescued.

Expression of ND-42^Scer\UAS.cMa under the control of Scer\GAL4^da.PU rescues the reduction in complex I seen in Pink1^B9 mutant flies.

(Pogson et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ND-42^Scer\UAS.cMa

ND-42^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Morais

Secondary FlyBase IDs

References (2)

Report Sections

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