FlyBase Allele Report: Dmel\ND-42[UAS.cBa]

General Information

Symbol

Dmel\ND-42^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Bellen

FlyBase ID

FBal0302431

Feature type

allele

Associated gene

Dmel\ND-42

Associated Insertion(s)

Carried in Construct

P{UAS-ND-42.B}

Key Links

Transgenic product class

wild_type

(Pogson et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Pogson et al., 2014)

Mutagen

in vitro construct

(Pogson et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-ND-42.B}

Cytology

Description

UAS regulatory sequences drive expression of ND-42.

(Pogson et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ND-42

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate Parkinson's disease

modeled by park²⁵

(Pogson et al., 2014)

ameliorates Parkinson's disease

modeled by Pink1^B9

(Pogson et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of ND-42^Scer\UAS.cBa under the control of Scer\GAL4^da.PU has no effect on climbing or flight behavior.

(Pogson et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/ND-42^UAS.cBa is a suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Pogson et al., 2014)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a suppressor of abnormal flight phenotype of Pink1^B9

(Pogson et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of abnormal locomotor behavior phenotype of Pink1^B9

(Tsai et al., 2018)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of abnormal flight phenotype of park²⁵

(Pogson et al., 2014)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of male sterile phenotype of park²⁵

(Pogson et al., 2014)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of male sterile phenotype of Pink1^B9

(Pogson et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/ND-42^UAS.cBa is a suppressor of flight muscle cell phenotype of Pink1^B9

(Pogson et al., 2014)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a suppressor of mitochondrion phenotype of Pink1^B9

(Pogson et al., 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of mitochondrion | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of mitochondrial crista | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of embryonic/larval hypodermal muscle cell | third instar larval stage phenotype of Pink1⁵

(Tsai et al., 2018)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of flight muscle cell phenotype of park²⁵

(Pogson et al., 2014)

Scer\GAL4^da.PU/ND-42^UAS.cBa is a non-suppressor of mitochondrion phenotype of park²⁵

(Pogson et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Ubiquitous expression of ND-42^Scer\UAS.cBa under the control of Scer\GAL4^da.PU restores climbing and flight ability in Pink1^B9 mutant flies. Flight muscle and mitochondrial integrity are only partially restored. Male sterility is not improved.

Ubiquitous expression of ND-42^Scer\UAS.cBa under the control of Scer\GAL4^da.PU does not rescue the climbing and flight defects seen in park²⁵ mutant flies. The flight muscle and mitochondrial integrity defects and male sterility are also not rescued.

Expression of ND-42^Scer\UAS.cBa under the control of Scer\GAL4^da.PU rescues the reduction in complex I and ATP levels seen in Pink1^B9 mutant flies.

Expression of ND-42^Scer\UAS.cBa under the control of Scer\GAL4^da.PU does not rescue the reduction in ATP levels seen in park²⁵ mutant flies. Complex I levels remain unchanged from wild type.

(Pogson et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ND-42^Scer\UAS.cBa

ND-42^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Bellen

Secondary FlyBase IDs

References (2)

Report Sections

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