FB2026_02 , released June 18, 2026
Allele: Dmel\tbrd-12
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General Information
Symbol
Dmel\tbrd-12
Species
D. melanogaster
Name
FlyBase ID
FBal0302630
Feature type
allele
Associated gene
Dmel\tbrd-1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Kimura and Loppin, 2015)
Mutagen
Delta2-3 transposase
(Kimura and Loppin, 2015)
Progenitor genotype
P{GSV6}GS13976
(Kimura and Loppin, 2015)
Cytology
Description

Imprecise excision of the P{GSV6} element in P{GSV6}GS13976 has resulted in a 1940bp deletion that extends from 650bp upstream to 1290bp downstream of the putative transcription start site of tbrd-1.

(Kimura and Loppin, 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:23,892,093..23,894,033 [-]
Comment:

A 1948 bp deletion resulting from the imprecise excision of P{GSV6}GS13976, which removes sequences from 650 bp upstream to 1290 bp downstream of the tbrd-1 putative transcription start site.

(Kimura and Loppin, 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In tbrd-12 mutant testes the post-meiotic spermatid nuclei at the onion stage contain nuclei of various sizes associated with irregularly shaped mitochondria derivatives. After meiosis, although some spermatid cysts start to elongate they appear abnormal in shape and contain aberrant nuclei scattered throughout their length, in contrast to wild type.

      (Kimura and Loppin, 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      tbrd-12 has spermatid phenotype, non-enhanceable by tbrd-2HR1

      (Kimura and Loppin, 2015)
      NOT Enhancer of
      Statement
      Reference

      tbrd-12 is a non-enhancer of spermatid phenotype of tbrd-2HR1

      (Kimura and Loppin, 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The spermatid phenotype observed in tbrd-12 tbrd-2HR double mutants is similar to that seen in either mutant alone. Primary spermatocyte morphology at earlier stages is also unaffected.

      (Kimura and Loppin, 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      tbrd-12 is rescued by tbrd-1+tKa

      (Kimura and Loppin, 2015)

      tbrd-12 is rescued by tbrd-1mRFP1,Tag:FLAG

      (Kimura and Loppin, 2015)
      Comments

      Expression of tbrd-1+tKa rescues the sterility seen in tbrd-12 mutant males.

      Expression of tbrd-1T:Disc\RFP-mRFP,T:Zzzz\FLAG rescues the sterility seen in tbrd-12 mutant males.

      (Kimura and Loppin, 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      tbrd-12
      (Kimura and Loppin, 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)