FlyBase Allele Report: Dmel\His4[HisTA.H4K20A]

General Information

Symbol

Dmel\His4^HisTA.H4K20A

Species

D. melanogaster

Name

FlyBase ID

FBal0304397

Feature type

allele

Associated gene

His4

Associated Insertion(s)

Carried in Construct

PBac{12xHisTA.H4K20A}

Key Links

Transgenic product class

missense_variant

(McKay et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(McKay et al., 2015)

Mutagen

in vitro construct

(McKay et al., 2015)

Progenitor genotype

Carried in construct

PBac{12xHisTA.H4K20A}

Cytology

Description

Mutated His4 gene (amino acid replacement K20A) in the context of a histone gene repeat unit.

(McKay et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

His4

Encoded product / tool

His4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of a transgene bearing 12 copies of a mutated form of the histone repeat unit (PBac{12xHisTA.H4K20A} - comprised of 12xHis4^HisTA.H4K20A and 12x wild type copies of His1^HisTA.wt, His2A^HisTA.wt, His2B^HisTA.wt and His3^HisTA.wt) in a wild type background has no observable phenotype.

(McKay et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Df(2L)His^C, His4^HisTA.H4K20A has increased mortality during development phenotype, enhanceable by His4r^15-4/His4r^S9

(Crain et al., 2022)

Df(2L)His^C, His4^HisTA.H4K20A has increased mortality during development phenotype, enhanceable by His4r^15-4/His4r^K20A

(Crain et al., 2022)

Other

Statement

Reference

Df(2L)His^C, His4^HisTA.H4K20A has partially lethal - majority die phenotype

(Crain et al., 2022)

Df(2L)His^C, His4^HisTA.H4K20A has decreased body size | pupal stage phenotype

(Crain et al., 2022)

Df(2L)His^C, His4^HisTA.H4K20A has majority die during P-stage phenotype

(Crain et al., 2022)

Df(2L)His^C, His4^HisTA.H4K20A, His4r^15-4/His4r^K20A has lethal - all die before end of P-stage phenotype

(Crain et al., 2022)

Df(2L)His^C, His4^HisTA.H4K20A, His4r^15-4/His4r^K20A has majority die during larval stage phenotype

(Crain et al., 2022)

Df(2L)His^C, His4^HisTA.H4K20A, His4r^LL05512 has partially lethal - majority live phenotype

(McKay et al., 2015)

Df(2L)His^C, His4^HisTA.H4K20A, His4r^LL05512 has some die during pupal stage phenotype

(McKay et al., 2015)

Phenotype Manifest In

Other

Statement

Reference

Df(2L)His^C, His4^HisTA.H4K20A, His4r^15-4 has interommatidial bristle phenotype

(Crain et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

85% of flies that are homozygous for His4r^LL05512 and Df(2L)His^C and express a mutated form of the histone repeat unit transgene containing 12 copies of the histone gene repeat unit (PBac{12xHisTA.H4K20A} - comprised of His4^HisTA.H4K20A and wild type copies of His1^HisTA.wt, His2A^HisTA.wt, His2B^HisTA.wt, His3^HisTA.wt) are able to pupate and 35% develop until late pharate adults.

(McKay et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

His4^HisTA.H4K20A partially rescues Df(2L)His^C

(McKay et al., 2015)

Comments

Expression of a transgene bearing 12 copies of a mutated form of the histone repeat unit (PBac{12xHisTA.H4K20A} -comprised of 12xHis4^HisTA.H4K20A and 12x wild type copies of His1^HisTA.wt, His2A^HisTA.wt, His2B^HisTA.wt and His3^HisTA.wt) partially rescues the lethality of homozygous Df(2L)His^C mutant flies. The resulting flies exhibit a significant developmental delay.

(McKay et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

H4^K20A

(Crain et al., 2024)

His4^HisTA.H4K20A

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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