FlyBase Allele Report: Hsap\RELA[AD.R33E02]

General Information

Symbol

Hsap\RELA^AD.R33E02

Species

H. sapiens

Name

FlyBase ID

FBal0304513

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R33E02-p65.AD}

PBac{R33E02-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R33E02-p65.AD}

PBac{R33E02-p65.AD}

Cytology

Description

The R33E02 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR33E02

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mushroom body output neuron 18 | faint

Comment: when combined with P{R50A05-GAL4.DBD} (combination referred to as 'MB080C')

(Aso et al., 2014)

adult lateral horn PV6a1 neuron

Comment: when combined with P{R47B03-GAL4.DBD} (combination referred to as 'LH320')

(Dolan et al., 2019)

adult lateral horn PV6a3 neuron

Comment: when combined with P{R47B03-GAL4.DBD} (combination referred to as 'LH320')

(Dolan et al., 2019)

mushroom body output neuron 18

Comment: when combined with P{R50A05-GAL4.DBD} (combination referred to as 'MB080C')

(Scaplen et al., 2021)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Blocking protein synthesis (using Rcom\RA[cs2.UAS] at 30[o]C) for the full 24 h between spaced training and testing in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R50A05 and Hsap\RELA^AD.R33E02, does not significantly affect aversive olfactory long term memory in adults, compared to wild-type controls.

(Wu et al., 2017)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R50A05 and Hsap\RELA^AD.R33E02 in adult males does not significantly affect the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

(Sitaraman et al., 2015)

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R50A05 and Hsap\RELA^AD.R33E02 in adults does not affect social or locomotor behaviors, compared to controls.

(Aso et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions