FlyBase Allele Report: Hsap\RELA[AD.R53C03]

General Information

Symbol

Hsap\RELA^AD.R53C03

Species

H. sapiens

Name

FlyBase ID

FBal0304521

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R53C03-p65.AD}

PBac{R53C03-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Aso et al., 2014)

Progenitor genotype

Carried in construct

P{R53C03-p65.AD}

PBac{R53C03-p65.AD}

Cytology

Description

The R53C03 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain). A Hsp70 transcriptional terminator is present.

(Aso et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

GMR53C03

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mushroom body output neuron 5

Comment: when combined with P{R24E12-GAL4.DBD} (combination referred to as 'MB298B')

(Ichinose et al., 2021, Scaplen et al., 2021, Aso et al., 2014, Aso et al., 2014)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R24E12 and Hsap\RELA^AD.R53C03 in adult males significantly reduces the amount of sleep, compared to controls; activation does not significantly affect locomotor activity of awake adult males, compared to controls.

(Sitaraman et al., 2015)

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R24E12 and Hsap\RELA^AD.R53C03 in adult females in an area of an arena significantly increases avoidance of that area, compared to controls.

Inhibition (using shi^{ts1.IVS.p10.20xUAS} at 31-33[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R24E12 and Hsap\RELA^AD.R53C03 in adults during training, consolidation and testing does not substantially reduce 2 h associative aversive or attractive olfactory memory, compared to controls; inhibition during testing alone does not significantly reduce 24 h associative aversive olfactory memory, compared to controls; inhibition only during training and testing significantly reduces 24 h appetitive, but not 30 min aversive, associative olfactory memory towards ethanol, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa or TrpA1^{20xUAS.JFRC124} at 28.5[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R24E12 and Hsap\RELA^AD.R53C03 in adults significantly reduces sleep, compared to controls, with no effect on social or locomotor behaviors.

(Aso et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions