FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\NetoUAS.cSa
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General Information
Symbol
Dmel\NetoUAS.cSa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Serpe
FlyBase ID
FBal0304565
Feature type
allele
Associated gene
Dmel\Neto
Associated Insertion(s)
Carried in Construct
P{UAS-Neto.S}
Key Links
Transgenic product class
wild_type
(Sulkowski et al., 2014)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Sulkowski et al., 2014)
Mutagen
in vitro construct
(Sulkowski et al., 2014)
Progenitor genotype
Carried in construct
P{UAS-Neto.S}
Cytology
Description

UAS regulatory sequences drive expression of Neto.

(Sulkowski et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Neto
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of NetoScer\UAS.cSa in a wild type background under the control of Scer\GAL4G14 results in a reduction in NMJ bouton number and shorter branches. Expression in a mutant background (Neto36) also results in a dose-dependent reduction in bouton number and branching: the higher the expression level of the transgene the more severe the phenotype. Overexpression of Neto also has detrimental effects on the viability of rescued animals at all stages of development. There is a reduction in mEJP frequency compared to controls and a small reduction in amplitude when NetoScer\UAS.cSa is expressed at very high levels. EJP amplitude is also reduced at high expression levels.

      (Kim et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4G14/NetoUAS.cSa rescues Neto36

      (Ramos et al., 2015)
      Comments

      Expression of NetoScer\UAS.cSa in muscles under the control of Scer\GAL4G14 rescues the embryonic lethality and paralysis seen in Neto36 mutants. Neto36 mutant flies expressing low/moderate levels of Scer\GAL4G14 under the control of Scer\GAL4G14 have significantly reduced spontaneous miniature excitatory junctional potential (mEJP) amplitudes. mEJP frequency is largely normal. Quantal content is significantly increased and evoked EJP is normal.

      (Ramos et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      NetoScer\UAS.cSa
      NetoUAS.cSa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Serpe
      Secondary FlyBase IDs
        References (4)