FlyBase Allele Report: Dmel\spri[RNAi.UAS]

General Information

Symbol

Dmel\spri^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0304743

Feature type

allele

Associated gene

Dmel\spri

Associated Insertion(s)

Carried in Construct

P{UAS-spri.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Sakuma et al., 2014)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Sakuma et al., 2014)

Mutagen

in vitro construct

(Sakuma et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-spri.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of a short-hairpin RNA (shRNA) directed against spri.

(Sakuma et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

spri

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Single-cell DL1 olfactory projection neuron clones expressing spri^{dsRNA.shRNA.Scer\UAS} under the control of Scer\GAL4^GH146 do not show obvious defects in axon elongation of dendrite branching.

(Sakuma et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^GH146/spri^RNAi.UAS is an enhancer | somatic clone of abnormal neuroanatomy | somatic clone phenotype of Strip^dogi

(Sakuma et al., 2014)

Other

Statement

Reference

DCTN1-p150^Gl-1, Scer\GAL4^GH146, spri^RNAi.UAS has abnormal neuroanatomy | somatic clone phenotype

(Sakuma et al., 2014)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^GH146/spri^RNAi.UAS is an enhancer | somatic clone of adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype of Strip^dogi

(Sakuma et al., 2014)

Other

Statement

Reference

DCTN1-p150^Gl-1, Scer\GAL4^GH146, spri^RNAi.UAS has adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype

(Sakuma et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The axon elongation and dendrite branching defects seen in Strip^dogi DL1 olfactory projection neuron single-cell clones are more severe if the cells are also expressing spri^{dsRNA.shRNA.Scer\UAS} under the control of Scer\GAL4^GH146. The axon fails to exit the antennal lobe in 13.0% of cases.

Gl¹ DL1 olfactory projection neuron single-cell clones expressing spri^{dsRNA.shRNA.Scer\UAS} under the control of Scer\GAL4^GH146 show axon elongation and dendrite branching defects. The axon fails to exit the antennal lobe in 38.9% of cases.

(Sakuma et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

spri^RNAi.UAS

spri^{dsRNA.shRNA.Scer\UAS}

spri^{dsRNA.shRNA.UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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