FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Act57BK328Q.UAS
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General Information
Symbol
Dmel\Act57BK328Q.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0304842
Feature type
allele
Associated gene
Dmel\Act57B
Associated Insertion(s)
Carried in Construct
P{UAS-Act57B.K328Q}
Key Links
Transgenic product class
missense_variant
(Viswanathan et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Viswanathan et al., 2015)
Mutagen
in vitro construct
(Viswanathan et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Act57B.K328Q}
Cytology
Description

UASt regulatory sequences drive expression of an acetyl-mimetic mutant of Act57B carrying amino acid substitution K328Q.

(Viswanathan et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Act57B
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Scer\GAL4Mef2.PR>Act57BK328Q.Scer\UAS expression eliminates flight ability. The flies show a characteristic "wings up" phenotype commonly associated with hypercontractile and damaged indirect flight muscles (IFMs). Indeed, polarized light microscopy reveals the absence of continuous IFM fibers.

      Flies expressing Scer\GAL4Mef2.PR>Act57BK328Q.Scer\UAS have strikingly reduced climbing capabilities compared with wild-type.

      Scer\GAL4Act88F.PB-mediated expression of Act57BK328Q.Scer\UAS causes a complete loss of flight. These flies show prominently hypercontracted IFM fibers, characterized by separation and accumulation of fiber material at one or both attachment sites. The phenotype is progressive and IFMs deteriorate with age.

      (Viswanathan et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      Act57BK328Q.UAS, Scer\GAL4Mef2.PR has indirect flight muscle cell phenotype, suppressible | partially by Mhc[+]/Mhc10

      (Viswanathan et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Scer\GAL4Mef2.PR>Act57BK328Q.Scer\UAS; Mhc10/+ flies display a greater abundance of thoracic material and birefringent musculature indicating partial suppression of the indirect flight muscle (IFM) phenotype of Scer\GAL4Mef2.PR>Act57BK328Q.Scer\UAS.

      (Viswanathan et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Act57BK328Q.Scer\UAS
      Act57BK328Q.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)