FlyBase Allele Report: Dmel\AhcyL2[HMJ23469]

General Information

Symbol

Dmel\AhcyL2^HMJ23469

Species

D. melanogaster

Name

FlyBase ID

FBal0315611

Feature type

allele

Associated gene

Dmel\AhcyL2

Associated Insertion(s)

Carried in Construct

P{TRiP.HMJ23469}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.HMJ23469}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMJ23469

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

long lived | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

(Parkhitko et al., 2016)

long lived | RU486 conditional, with Scer\GAL4^TIGS-2

(Parkhitko et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of AhcyL2^HMJ23469 driven by Scer\GAL4^{Act5C.Switch.PR} (in males fed RU486 during adulthood, to avoid developmental effects) significantly extends lifespan compared to controls. Expression of AhcyL2^HMJ23469 driven simultaneously by Scer\GAL4^{bun-Switch1.32} and Scer\GAL4^Switch1.106 or by Scer\GAL4^{elav.Switch.PO} (in males fed RU486 during adulthood) does not significantly affect life span compared to controls; when expression is driven by Scer\GAL4^TIGS-2, there is a significant extension of lifespan in RU486-fed females.