FlyBase Allele Report: Dmel\cnn[S22A.T27A.PA.UASp.EGFP]

General Information

Symbol

Dmel\cnn^{S22A.T27A.PA.UASp.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0317022

Feature type

allele

Associated gene

Dmel\cnn

Associated Insertion(s)

Carried in Construct

P{UASp-cnn.PA.S22A.T27A.eGFP}

Key Links

Transgenic product class

missense_variant

(Eisman et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Eisman et al., 2015)

Mutagen

in vitro construct

(Eisman et al., 2015)

Progenitor genotype

Carried in construct

P{UASp-cnn.PA.S22A.T27A.eGFP}

Cytology

Description

UASp regulatory sequences drive expression of cnn-PA carrying amino acid substitutions S22A and T27A and tagged with EGFP.

(Eisman et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

cnn

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Df(2R)cnn/cnn^HK21, Scer\GAL4^{VP16.nanos.UTR}

(Eisman et al., 2015)

partially lethal - majority die | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, cnn^mfs3

(Eisman et al., 2015)

Phenotype Manifest In

nucleus | embryonic stage, with Df(2R)cnn/cnn^HK21, Scer\GAL4^{VP16.nanos.UTR}

(Eisman et al., 2015)

Detailed Description

Statement

Reference

The most common nuclear type in prophase stage embryos expressing cnn^{S22A.T27A.PA.Scer\UAS.P\T.T:Avic\EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in a cnn^HK21/Df(2R)cnn mutant genetic background appears to be haploid with most nuclei located at the cortex, but deep divisions are still observed in less than 5% of animals. Polar body formation or persistent meiotic-like spindle is not detected in the mutant embryos. Development fails well before cellularization in the transgenic animals.

The number of adults that develop to adulthood represents only 14% of eggs laid by cnn^mfs3 mutant females that also express cnn^{S22A.T27A.PA.Scer\UAS.P\T.T:Avic\EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Eisman et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

cnn^{PA.Scer\UAS.P\T.T:Avic\EGFP\S22A\T27A}

(Eisman et al., 2015)

cnn^{S22A.T27A.PA.Scer\UAS.P\T.T:Avic\EGFP}

cnn^{S22A.T27A.PA.UASp.EGFP}

cnn^{S22A.T27A.PA.UASp.T:Avic\EGFP}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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