FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Ddx1AX
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General Information
Symbol
Dmel\Ddx1AX
Species
D. melanogaster
Name
FlyBase ID
FBal0318059
Feature type
allele
Associated gene
Dmel\Ddx1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - molecular evidence

(Germain et al., 2015)
Mutagen
Delta2-3 transposase
(Germain et al., 2015)
Progenitor genotype
P{EPgy2}EY12792
(Germain et al., 2015)
Cytology
Description

Imprecise excision of the progenitor P{EPgy2}EY12792 insertion, resulting in a 1733bp deletion extending from -43 to +1689bp relative to the Ddx1 transcription start site (coordinates 3L:22263326..22265059 , release 6 genome), which encompasses the majority of the open reading frame. A 15bp sequence (5'-CATGATGAAATAACA-3') is present in place of the deleted sequence. This 15bp does not correspond to any part of the Ddx1 locus or the original inserted P{EPgy2} element.

(Germain et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:22,263,326..22,265,059 [+]
Comment:

A 1733bp deletion resulting from imprecise excision of the progenitor P{EPgy2}EY12792 insertion, which extends from -43 to +1689bp relative to the Ddx1 transcription start site (coordinates 3L:22263326..22265059 , release 6 genome).

(Germain et al., 2015)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Homozygous adult flies are viable, but they are out-competed by their heterozygous siblings unless raised under low density culturing conditions.

      Homozygous and Ddx1AX/Df(3L)ED230 males are completely sterile. Homozygous females are completely sterile, while Ddx1AX/Df(3L)ED230 females are able to produce a small number of offspring that survive to adulthood.

      Homozygotes show a significant delay in eclosion compared to heterozygous controls. A trend towards later pupation is also seen in the homozygotes, but this trend is not significant.

      Homozygous and Ddx1AX/Df(3L)ED230 pupae and adults are significantly shorter than wild-type or heterozygous controls. Homozygous pupae are slightly, but significantly, shorter than Ddx1AX/Df(3L)ED230 pupae.

      The ovaries of 3 day old homozygous and Ddx1AX/Df(3L)ED230 females are much smaller than normal and either do not contain mature eggs (homozygotes) or contain only a small number of mature eggs (Ddx1AX/Df(3L)ED230 females). The ovaries of 10 day old homozygous females contain a small number of abnormal eggs that lack dorsal appendages. Mid-stage autophagic egg chambers are seen at both 3 and 10 days post eclosion in homozygous and Ddx1AX/Df(3L)ED230 ovaries.

      Early stage egg chambers and germ-line stem cell niches appear normal in homozygous and Ddx1AX/Df(3L)ED230 females.

      Early spermatogenesis appears normal in homozygous and Ddx1AX/Df(3L)ED230 males, with developing spermatids undergoing nuclear elongation. However, elongated spermatids are disrupted and scattered. No mature sperm is seen in the seminal vesicles of homozygous males.

      (Germain et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of SirupGD14644 under the control of Scer\GAL4Act5C.PU in both a Ddx1AX/+ and a Ddx1AX/Ddx1AX background results in almost completely lethality.

      (Germain et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Ddx1AX
      (Germain et al., 2015)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)