FlyBase Allele Report: Dmel\ADD1[NP1240]

General Information

Symbol

Dmel\ADD1^NP1240

Species

D. melanogaster

Name

FlyBase ID

FBal0318460

Feature type

allele

Associated gene

Dmel\ADD1

Associated Insertion(s)

P{GawB}ADD1^NP1240

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(López-Falcón et al., 2014)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}ADD1^NP1240

Cytology

Description

Insertion in the 5'UTR of ADD1 which results in reduced levels of ADD1 mRNA compared to wild type. Insertion is at +83bp relative to the transcription start site and at -223bp relative to the translation initiation codon.

(López-Falcón et al., 2014)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:11,970,606..11,970,606 [-]

(López-Falcón et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile (with ADD1^NP0793)

(López-Falcón et al., 2014)

partially lethal - majority die

(López-Falcón et al., 2014)

partially lethal - majority live (with ADD1^NP0793)

(López-Falcón et al., 2014)

suppressor of variegation, with In(3L)BL1

(López-Falcón et al., 2014)

Phenotype Manifest In

Detailed Description

Statement

Reference

ADD1^NP1240/ADD1^NP1240 or ADD1^NP0793/ADD1^NP1240 flies have reduced viability and no melanotic masses (ADD1^NP1240/+ or ADD1^NP0793/+ flies are viable and have no melanotic masses).

ADD1^NP1240 suppresses variegation of the Ecol\lacZ reporter carried on In(3L)BL1 (with a role in maintenance rather than establishment of heterochromatin).

(López-Falcón et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

ADD1[+]/ADD1^NP1240, Su(var)205², XNP³/XNP[+] has melanotic mass phenotype | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP¹/XNP³ has melanotic mass phenotype | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP²/XNP³ has melanotic mass phenotype | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP²/XNP¹ has melanotic mass phenotype | larval stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP¹/XNP[+] has melanotic mass phenotype | larval stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP¹/XNP[+] has melanotic mass phenotype | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP²/XNP[+] has melanotic mass phenotype | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP³/XNP[+] has melanotic mass phenotype | adult stage phenotype

(López-Falcón et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

ADD1[+]/ADD1^NP1240, Su(var)205², XNP³/XNP[+] has melanotic mass | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP¹/XNP³ has melanotic mass | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP²/XNP³ has melanotic mass | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP²/XNP¹ has melanotic mass | larval stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP¹/XNP[+] has melanotic mass | larval stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP¹/XNP[+] has melanotic mass | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP²/XNP[+] has melanotic mass | adult stage phenotype

(López-Falcón et al., 2014)

ADD1^NP1240, XNP³/XNP[+] has melanotic mass | adult stage phenotype

(López-Falcón et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

ADD1^NP1240/+;XNP¹/+, ADD1^NP1240/+;XNP²/+, or ADD1^NP1240/+;XNP³/+ flies are viable and a small percentage of adults (7%, 2%, 2%) have melanotic masses.

ADD1^NP1240/+;XNP¹/XNP³ or ADD1^NP1240/+;XNP²/XNP³ flies show partial lethality (more flies survive than in XNP¹/XNP³ or XNP²/XNP³ combinations) and have melanotic masses (12%, 8%).

(López-Falcón et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Kyoto

103952

y^* w^*; P{GawB}ADD1^NP1240 / CyO, P{UAS-lacZ.UW14}UW14

112583

y^* w^*; P{GawB}ADD1^NP1240 / CyO, P{UAS-lacZ.UW14}UW14

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ADD1^NP1240

dadd1^NP1240

(López-Falcón et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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