Wild type males mated to Nep2^Δ females lay significantly fewer eggs than controls. The proportion of progeny that eclose from laid eggs (hatchability) is also greatly reduced.

There is no difference in fertilization rate between eggs laid by Nep2^Δ females, but significantly more of the laid eggs arrest in very early embryogenesis.

After mating to wild type males, Nep2^Δ females store more sperm overall at 2 hours post-mating, but store fewer sperm at 4 hours post-mating, compared to controls. This indicates a defect in sperm retention. Also, Nep2^Δ females sire fewer progeny from first male matings than second male mating (compared to controls), indicating a defect in female-regulated sperm use.