FlyBase Allele Report: Zzzz\CTG[130.UAS.DsRed2]

General Information

Symbol

Zzzz\CTG^{130.UAS.DsRed2}

Species

a. artificial

Name

FlyBase ID

FBal0319639

Feature type

allele

Associated gene

Zzzz\CTG

Associated Insertion(s)

Carried in Construct

P{UAS-DsRed-CTG130}

Transgenic product class

trinucleotide_repeat_expansion

(Yu et al., 2011)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

trinucleotide_repeat_expansion

(Yu et al., 2011)

Mutagen

in vitro construct

(Yu et al., 2011)

Progenitor genotype

Carried in construct

P{UAS-DsRed-CTG130}

Cytology

Description

UASt regulatory sequences drive expression of DsRed2 coding sequences, with a CTG sequence repeated an uninterrupted 130 times in the 3'UTR.

(Yu et al., 2011)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Zzzz\CTG

Tagged with

DsRed2

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Yu et al., 2011)

DMPK:n.3_prime_UTR[130CUG]

(FlyBase curators, 2019-)

Variants Synonym(s)

DMPK, (CTG)n REPEAT EXPANSION, 3-PRIME UTR

DMPK:n.3_prime_UTR[130CTG]

Associated human disease model(s)

myotonic dystrophy 1

External database links

ClinVar: 5049

Comments concerning this variant

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PF

(Yu et al., 2011)

increased mortality during development, with Scer\GAL4^how-24B, Zzzz\CAG^{100.UAS.DsRed2}

(Yu et al., 2011)

viable, with Scer\GAL4^da.G32

(Yu et al., 2011)

viable, with Scer\GAL4^elav-C155

(Yu et al., 2011)

viable, with Scer\GAL4^how-24B

(Yu et al., 2011)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Yu et al., 2011)

retina, with Scer\GAL4^GMR.PF

(Yu et al., 2011)

Detailed Description

Statement

Reference

Expression of Disc\RFP^{(CTG)130.Scer\UAS} in the muscle (Scer\GAL4^how-24B), nervous system (Scer\GAL4^elav-C155) or ubiquitously (Scer\GAL4^da.G32) does not affect viability.

Expression of Disc\RFP^{(CTG)130.Scer\UAS} in the developing eye, under the control of Scer\GAL4^GMR.PF gives a mild rough eye phenotype, with abnormal eye pigmentation and disruption of retinal integrity.

Co-expression of Disc\RFP^{CAG100.DsRed2.Scer\UAS} with Disc\RFP^{(CTG)130.Scer\UAS} in the muscle, under the control of Scer\GAL4^how-24B, results in developmental lethality.

Expression of Disc\RFP^{(CTG)130.Scer\UAS} in the muscle, under the control of Scer\GAL4^how-24B, does not generate a phenotype.

(Yu et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^how-24B, Zzzz\CAG^{100.UAS.DsRed2}, Zzzz\CTG^{130.UAS.DsRed2} has increased mortality during development phenotype, suppressible by Dcr-2^L811fsX

(Yu et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

A Dcr-2^L811fsX homozygous mutant background suppresses the developmental lethality associated with expression of Disc\RFP^{(CTG)130.Scer\UAS} and Disc\RFP^{CAG100.DsRed2.Scer\UAS} in the developing muscle (under the control of Scer\GAL4^how-24B)

(Yu et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

79577

w¹¹¹⁸; P{UAS-DsRed-CTG130}1.3/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Disc\RFP^{(CTG)130.Scer\UAS}

Zzzz\CTG^{130.Scer\UAS.T:Disc\RFP}

Zzzz\CTG^{130.UAS.DsRed2}

Name Synonyms

Secondary FlyBase IDs

FBal0319584
FBal0285125

References (3)

Report Sections

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