FlyBase Allele Report: Dmel\Prosap[D101]

General Information

Symbol

Dmel\Prosap^D101

Species

D. melanogaster

Name

FlyBase ID

FBal0319808

Feature type

allele

Associated gene

Dmel\Prosap

Associated Insertion(s)

Carried in Construct

Also Known As

Shank^D101

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

minos activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Harris et al., 2016)

Mutagen

minos activity

(Harris et al., 2016)

Progenitor genotype

Mi{ET1}Prosap^MB03234

(Harris et al., 2016)

Cytology

Description

Deletion that extends from the middle of the first Prosap intron to the 3'UTR (deletion corresponds to 2R:14062907..14074533 , release 6 genome). Generated by imprecise excision of Mi{ET1}Prosap^MB03234.

(Harris et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:14,062,907..14,074,533 [-]

Comment:

Deletion reported as mapping to 2R:14062907..14074533 , release 6 genome resulting from the imprecise excision of Mi{ET1}Prosap^MB03234.

(Harris et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | dominant | larval stage

(Wu et al., 2017)

abnormal neuroanatomy | dominant | third instar larval stage

(Harris et al., 2016)

abnormal neuroanatomy | larval stage (with Df(2R)8328)

(Wu et al., 2017)

abnormal neuroanatomy | larval stage (with Prosap^8k)

(Wu et al., 2017)

abnormal neuroanatomy | third instar larval stage (with Df(2R)BSC361)

(Harris et al., 2016)

abnormal size (with Df(2R)8328)

(Wu et al., 2017)

abnormal size (with Prosap^8k)

(Wu et al., 2017)

abnormal size | dominant | larval stage

(Wu et al., 2017)

viable

(Harris et al., 2016)

Phenotype Manifest In

antennal lobe projection neuron

(Wu et al., 2017)

antennal lobe projection neuron (with Df(2R)8328)

(Wu et al., 2017)

antennal lobe projection neuron | larval stage (with Prosap^8k)

(Wu et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage

(Harris et al., 2016)

embryonic/larval neuromuscular junction | third instar larval stage (with Df(2R)BSC361)

(Harris et al., 2016)

larval mushroom body calyx glomerulus

(Wu et al., 2017)

larval mushroom body calyx glomerulus (with Df(2R)8328)

(Wu et al., 2017)

larval mushroom body calyx glomerulus (with Prosap^8k)

(Wu et al., 2017)

NMJ bouton | third instar larval stage

(Harris et al., 2016)

NMJ bouton | third instar larval stage (with Df(2R)BSC361)

(Harris et al., 2016)

subsynaptic reticulum | third instar larval stage

(Harris et al., 2016)

terminal bouton

(Wu et al., 2017)

terminal bouton (with Df(2R)8328)

(Wu et al., 2017)

terminal bouton | larval stage (with Prosap^8k)

(Wu et al., 2017)

Detailed Description

Statement

Reference

The larval mushroom body calyx glomeruli of Prosap^D101 homozygotes and Prosap^D101/Prosap^8k or Prosap^D101/Df(2R)8328 transheterozygotes are significantly smaller and uni-lobed (with no apparent protrusion), as compared to the multi-lobed, highly protruded glomeruli of controls; all these mutant calyx glomeruli present a severe decrease in the number of antennal lobe projection neuron presynaptic bouton protrusions (labeled by ChAt), as compared to controls. Similar but milder defects (in both the overall glomerular morphology and number of presynaptic boutons) are also detected in Prosap^D101 heterozygotes.

(Wu et al., 2017)

Prosap^D101/Prosap^D101 mutants survive to adulthood, but examination of third instar NMJs at muscle 6/7 reveals a reduction in the number of synaptic boutons per NMJ, but no significant change in bouton size or microtubule organization in boutons, and an abnormally high number of Ghost boutons per NMJ, as compared with controls. Prosap^D101/Prosap^D101 mutants have normal presynaptic ultrastructure, but exhibit defects in the subsynaptic reticulum, including fewer membranous folds and decreased contact with the presynaptic terminal, but no change in overall area, as compared to controls.

NMJs at muscle 6/7 in third instar larvae of Prosap^D101/Df(2R)BSC361 mutants display a reduction in the number of synaptic boutons per NMJ, and an abnormally high number of Ghost boutons per NMJ, as compared with controls.

NMJs at muscle 6/7 in third instar larvae of Prosap^D101/+ mutants display a reduction in the number of synaptic boutons per NMJ, but no significant difference in number of Ghost boutons per NMJ, as compared with controls.

(Harris et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Prosap^D101 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Mef2.PR

(Harris et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Prosap^D101 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Mef2.PR

(Harris et al., 2016)

Prosap^D101 has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^Mef2.PR

(Harris et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of fz2^{C.Scer\UAS.NLS.T:Hsap\MYC} under the control of Scer\GAL4^Mef2.PR fully rescues the increase in Ghost boutons, and partially rescues the total bouton number defect seen in Prosap^D101/Prosap^D101 mutants.

(Harris et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Prosap^D101 is partially rescued by Scer\GAL4^how-24B/Prosap^UASp.cHa

(Harris et al., 2016)

Not rescued by

Prosap^D101 is not rescued by Scer\GAL4^Mef2.PR/Prosap^UASp.cHa

(Harris et al., 2016)

Prosap^D101 is not rescued by Scer\GAL4^elav-C155/Prosap^UASp.cHa

(Harris et al., 2016)

Comments

Expression of Prosap^{Scer\UAS.P\T.cHa} under the control of Scer\GAL4^Mef2.PR fails to rescue the morphology defects of Prosap^D101/Prosap^D101 mutants.

Expression of Prosap^{Scer\UAS.P\T.cHa} under the control of Scer\GAL4^how-24B partially rescues the total bouton number defect, and rescues the Ghost bouton number increase of Prosap^D101/Prosap^D101 mutants.

Expression of Prosap^{Scer\UAS.P\T.cHa} under the control of Scer\GAL4^elav-C155 fails to rescue the morphology defects of Prosap^D101/Prosap^D101 mutants.

(Harris et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Prosap^D101

Shank^D101

(Wu et al., 2017, Harris et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

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