FB2026_03 , released September 17, 2026
Allele: Dmel\nAChRα6G275E
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General Information
Symbol
Dmel\nAChRα6G275E
Species
D. melanogaster
Name
FlyBase ID
FBal0319978
Feature type
allele
Associated gene
Dmel\nAChRα6
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Zimmer et al., 2016)
Progenitor genotype
Cytology
Description

Amino acid replacement: G275E.

(Zimmer et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
2L:9,798,304..9,798,305 [-]
Nucleotide change:

GC9798305AA

Amino acid change:

G301E | nAChRalpha6-PA; G301E | nAChRalpha6-PB; G301E | nAChRalpha6-PC; G316E | nAChRalpha6-PD; G330E | nAChRalpha6-PE; G301E | nAChRalpha6-PG; G301E | nAChRalpha6-PH

Reported amino acid change:

G275E

Comment:

2 base CRISPR mutation (GC to AA substiution) reported as G275E but mapping to G301E in FlyBase annotations nAChRalpha6-PA, PB, PC, PG, and PH. There is also a C to G substitution in the preceding intron at 2L:9798311 .

(Zimmer et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      nAChRα6G275E/nAChRα6G275E mutants have significantly reduced susceptibility to the insecticide spinosad, and nAChRα6G275E/+ mutants show a smaller reduction in susceptibility to spinosad than homozygotes, as compared with wild type.

      (Zimmer et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Dα6 G275E
      (Zimmer et al., 2016)
      nAChRα6G275E
      Name Synonyms
      Secondary FlyBase IDs
        References (1)