FlyBase Allele Report: Dmel\Git[ex10]

General Information

Symbol

Dmel\Git^ex10

Species

D. melanogaster

Name

FlyBase ID

FBal0320067

Feature type

allele

Associated gene

Dmel\Git

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

FLPase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Podufall et al., 2014)

Mutagen

FLPase

(Podufall et al., 2014)

Progenitor genotype

PBac{WH}Git^f03586

(Podufall et al., 2014)

P{XP}d02327

(Podufall et al., 2014)

Caused by aberration

Df(2R)Git^ex10

Cytology

Description

Deletion that removes the entire CG11883 gene and the 3' part of Git (including the Spa2 homology domain and paxillin-binding site domain). Generated by FLP-mediated recombination between the two progenitor insertions, PBac{WH}Git^f03586 and P{XP}d02327.

(Podufall et al., 2014)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:10,617,537..10,644,244 [-]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions, PBac{WH}Git^f03586 and P{XP}d02327, has resulted in the deletion of the genomic sequence between them.

(Podufall et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage (with Df(2R)BSC596)

(Podufall et al., 2014)

abnormal locomotor behavior | adult stage (with Git^f03586)

(Podufall et al., 2014)

abnormal neuroanatomy | third instar larval stage (with Git^f03586)

(Podufall et al., 2014)

abnormal neurophysiology | third instar larval stage (with Git^f03586)

(Podufall et al., 2014)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage (with Git^f03586)

(Podufall et al., 2014)

endosome | third instar larval stage (with Git^f03586)

(Podufall et al., 2014)

synaptic vesicle | third instar larval stage (with Git^f03586)

(Podufall et al., 2014)

Detailed Description

Statement

Reference

Git^ex10/Git^f03586 or Git^ex10/Df(2R)BSC596 flies have significant defects in climbing ability compared to controls.

Postsynaptic glutamate receptor fields are enlarged in neuromuscular junctions (NMJ) of Git^ex10/Git^f03586 third instar larvae. Git^ex10/Git^f03586 third instar larval NMJs (type 1b) are similar to controls in terms of synaptic vesicle number, docked vesicle number, active zone size or T-bar morphology; however, stimulated mutants display an accumulation of endosomal structures and vacuoles (>80nm vesicles or cisternae).

Git^ex10/Git^f03586 third instar larval NMJs show significant reductions in evoked excitatory junctional currents (eEJCs) compared to controls (no difference in rise times or decay constants).

Git^ex10/Git^f03586 or Git^ex10/Df(2R)BSC596 larval NMJs show defects in synaptic vesicle recycling, with Git^ex10/Git^f03586 mutants having specific defects in the slow phase of endocytosis and synaptic vesicle reacidification. Under conditions of elevated extracellular calcium, evoked neurotransmission of Git^ex10/Git^f03586 larvae is similar to controls but FM1-43 uptake is significantly reduced (impaired endocytosis or synaptic vesicle recycling).

(Podufall et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Git^f03586/Git^ex10 is rescued by Scer\GAL4^elav-C155/Git^UAS.cPa

(Podufall et al., 2014)

Git^f03586/Git^ex10 is rescued by Scer\GAL4^RapGAP1-OK6/Git^UAS.cPa

(Podufall et al., 2014)

Git^f03586/Git^ex10 is rescued by Scer\GAL4^elav-C155/Git^UAS.cPa.EGFP

(Podufall et al., 2014)

Git^ex10/Df(2R)BSC596 is rescued by Scer\GAL4^RapGAP1-OK6/Git^UAS.cPa

(Podufall et al., 2014)

Comments

Expression of Git^Scer\UAS.cPa or Git^{Scer\UAS.cPa.T:Avic\GFP-EGFP} driven by Scer\GAL4^elav-C155 rescues climbing defects seen in Git^ex10/Git^f03586 flies.

Expression of Git^Scer\UAS.cPa driven by Scer\GAL4^RapGAP1-OK6 rescues enlarged postsynaptic glutamate receptor fields and reduced eEJCs seen at the NMJ in Git^ex10/Git^f03586 third instar larvae. Expression of Git^Scer\UAS.cPa driven by Scer\GAL4^elav-C155 rescues increased endosome area per bouton seen at the NMJ in Git^ex10/Git^f03586 third instar larvae. Expression of Git^Scer\UAS.cPa or Git^{Scer\UAS.cPa.T:Avic\GFP-EGFP} driven by Scer\GAL4^RapGAP1-OK6 rescues synaptic vesicle recycling defects at the NMJ in Git^ex10/Git^f03586 or Git^ex10/Df(2R)BSC596 third instar larvae.

(Podufall et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Git^ex10

dgitex10

(Podufall et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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