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General Information
Symbol
TNPV\ank1UASp.cDa
Species
u. Toxoneuron nigriceps polydnavirus
Name
FlyBase ID
FBal0321203
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASp sequences drive expression of TNPV\ank1 cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Zzzz\ank1Scer\UAS.P\T.cDa using Scer\GAL4h-1J3 does not appear to affect embryonic and larval development, but larvae fail to pupate and die after an extended third instar larval life, which lasts up to three weeks. The size of the transgenic larvae expressing Zzzz\ank1Scer\UAS.P\T.cDa does not significantly differ from control larvae four days after egg deposition (AED). The transgenic larvae continue to feed and significantly increase in sized during their prolonged larval life, reaching at eighteen days AED the maximal length, while control animals pupated on day six AED.

When Zzzz\ank1Scer\UAS.P\T.cDa is expressed using Scer\GAL4P0206, larvae fail to pupate and show the same phenotype obtained with Scer\GAL4h-1J3.

When Zzzz\ank1Scer\UAS.P\T.cDa is expressed using Scer\GAL4Aug21, no effects on developmental timing is observed and regular progeny is obtained.

When Zzzz\ank1Scer\UAS.P\T.cDa is expressed using Scer\GAL4phm.PO, none of the larvae fail to pupate and they have en extended larval life as shown using Scer\GAL4P0206.

Ecdysteroid biosynthesis is impaired in larvae expressing Scer\GAL4phm.PO>Zzzz\ank1Scer\UAS.P\T.cDa. Transgenic third instar larvae fed with 20-hydroxyecdysone (20E) just before the onset of the ecdysteroid peak occurring in the wild-type partially rescues the developmental arrest phenotype. All the mutant larvae pupate, but they fail to progress to the pharate stage.

Prothoracic glands (PGs) from control larvae are significantly larger than PGs from Scer\GAL4phm.PO>Zzzz\ank1Scer\UAS.P\T.cDa larvae. The size difference can be attributed to a reduction in cell number.

Expression of Zzzz\ank1Scer\UAS.P\T.cDa using Scer\GAL4phm.PO causes disruption of the cytoskeletal network.

Expression of Zzzz\ank1Scer\UAS.P\T.cDa using Scer\GAL4phm.PO and Scer\GAL80ts.αTub84B engenders abnormal F-actin organisation at the restrictive temperature.

Germline expression of Zzzz\ank1Scer\UAS.P\T.cDa, under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 interferes with embryonic development. The expression of one copy of Zzzz\ank1Scer\UAS.P\T.cDa induces a maternal effect semi-lethal phenotype. Lethality of 56.3 and 42% is observed with two different lines, inserted on the X or on the II chromosome respectively.

Transgenic females that carry two copies of Zzzz\ank1Scer\UAS.P\T.cDa and one copy of Scer\GAL4nos.UTR.T:Hsim\VP16 exhibit 99.2% lethality.

Expression of two copies of Zzzz\ank1Scer\UAS.P\T.cDa and one copy of Scer\GAL4nos.UTR.T:Hsim\VP16 causes ventralization of the eggshell, with defects in dorsal appendages. The eggs also appear short and rounded. In some eggs the dorsal appendages are fused at the base. The majority of eggs (65%) exhibit the most extreme ventralized phenotype with the complete loss of all appendage material.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cHa with Scer\GAL4phm.PO>Zzzz\ank1Scer\UAS.P\T.cDa using Scer\GAL80ts.αTub84B fails to suppress the developmental arrest phenotype.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
TNPV\ank1Scer\UAS.P\T.cDa
TNPV\ank1UASp.cDa
Zzzz\ank1Scer\UAS.P\T.cDa
Name Synonyms
Secondary FlyBase IDs
  • FBal0320589
  • FBal0265166
References (3)