UASt regulatory sequences drive expression of full-length Dcaa\AFP-4 containing the native signal peptide sequence.
Transgenic flies expressing Zzzz\AFP-4Scer\UAS.cLa under the control of Scer\GAL4αTub84B.PL show higher survivorship at 0[o]C and 4[o]C than controls.
Transgenic flies co-expressing Zzzz\AFP-1Scer\UAS.cLa and Zzzz\AFP-4Scer\UAS.cLa under the control of Scer\GAL4αTub84B.PL show higher survivorship at 0[o]C and 4[o]C than controls.