UAS regulatory sequences drive expression of the bun A isoform. The protein is tagged at both ends with both Tag:SBP and the Tag:NLS(SV40-largeT) nuclear localisation signal.
Expression of bunA.Scer\UAS.T:Zzzz\SBP,T:SV40\nls-largeT under the control of Scer\GAL4esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) does not significantly affect proliferation or cell number in the midgut.
Co-expression of bunA.Scer\UAS.T:Zzzz\SBP,T:SV40\nls-largeT and MadmScer\UAS.cGa under the control of Scer\GAL4esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) does not significantly affect proliferation or cell number in the midgut.