FB2026_02 , released June 18, 2026
Allele: Dmel\TorsinRNAi.UASp
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General Information
Symbol
Dmel\TorsinRNAi.UASp
Species
D. melanogaster
Name
FlyBase ID
FBal0321718
Feature type
allele
Associated gene
Dmel\Torsin
Associated Insertion(s)
Carried in Construct
P{UAS-Torsin.RNAi.WIZ.M}
Key Links
Transgenic product class
RNAi_reagent
(Muraro and Moffat, 2006)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Muraro and Moffat, 2006)
Mutagen
in vitro construct
(Muraro and Moffat, 2006)
Progenitor genotype
Carried in construct
P{UAS-Torsin.RNAi.WIZ.M}
Cytology
Description

UASp sequences regulate expression of two copies of the second exon of Torsin cloned in a tail-to-tail configuration separated by a 70 bp spacer, which mediates RNAi against the Torsin transcript.

(Muraro and Moffat, 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASp
Encoded product / tool
Torsin
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of TorsindsRNA.Scer\UAS.P\T driven by Scer\GAL4GMR.PF results in a weak rough eye (disorganized retinas) phenotype compared to controls; additional presence of Df(1)bi-DL2 results in a slightly stronger phenotype (though Df(1)bi-DL2/+ alone does not have a rough eye phenotype). At 4 and 7 weeks old, Df(1)bi-DL2/+;Scer\GAL4GMR.PF>TorsindsRNA.Scer\UAS.P\T flies show a widespread significant increase in degenerative spots (holes) on the retina, compared to controls (holes are also significantly increased from 4 to 7 weeks old in mutants). Expression of TorsindsRNA.Scer\UAS.P\T driven by Scer\GAL4GMR.PF results in thickening of pigment granule areas in the retina (38% penetrance) and disorganized pigment granule patterns with occasional gaps or accumulations; remaining retinas do not show a clear thickening phenotype, but show disorganized granule patterns and additional presence of Df(1)bi-DL2 results in stronger phenotypes.

      (Muraro and Moffat, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TorsinRNAi.UASp
      TorsindsRNA.Scer\UAS.P\T
      TorsindsRNA.UASp
      Name Synonyms
      Secondary FlyBase IDs
        References (2)