FB2026_03 , released September 17, 2026
Allele: Dmel\lolaHA.miGFPi.PD
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General Information
Symbol
Dmel\lolaHA.miGFPi.PD
Species
D. melanogaster
Name
FlyBase ID
FBal0322748
Feature type
allele
Associated gene
Dmel\lola
Associated Insertion(s)
TI{TI}lolaHA.miGFPi.PD
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Wissel et al., 2016)
Progenitor genotype
Associated Insertion(s)
TI{TI}lolaHA.miGFPi.PD
Cytology
Description

A cassette comprised of two copies of the Tag:HA tag plus the Tag:miGFPi RNAi-effector sequence has been inserted in frame at the C-terminal end of the open reading frame of the lola-PD isoform. The position of the cassette allows for specific knockdown of the lola-PD isoform using an RNAi reagent that targets EGFP.

(Wissel et al., 2016)
Allele components
Component
Use(s)
Tagged with
Tag:HA
Tag:miGFPi
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 under the control of Scer\GAL4wor.PA in lolaHA.miGFPi.PD homozygotes to knock-down only the lola-PD isoform using the miGFPi method (tagging a gene with a minimal GFP tag and then expressing RNAi against the tag) does not cause overproliferation of neural stem cells in the larval central brain. Similarly, expression of Avic\GFPdsRNA.shRNA.Scer\UAS.1 in lolaV5.miGFPi.all/lolaV5.miGFPi.PD larvae (=effectively knocking down lola-PD only) also does not cause neuroblast overproliferation but when both lola-PD and lola-PB isoforms are knocked-down simultaneously in lolaV5.miGFPi.all/lolaV5.miGFPi.PD,lolaHA.miGFPi.PD larvae, neuroblast overproliferation is observed.

      (Wissel et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      lolaHA.miGFPi.PD
      Name Synonyms
      Secondary FlyBase IDs
        References (1)