FlyBase Allele Report: Dmel\Lpin[RNAi.hs]

General Information

Symbol

Dmel\Lpin^RNAi.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0324126

Feature type

allele

Associated gene

Dmel\Lpin

Associated Insertion(s)

Carried in Construct

P{hs-Lpin.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Schmitt et al., 2015)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Schmitt et al., 2015)

Mutagen

in vitro construct

(Schmitt et al., 2015)

Progenitor genotype

Carried in construct

P{hs-Lpin.RNAi}

Cytology

Description

A heat shock promoter drives expression of RNAi against Lpin (two PCR products derived from cDNA GH19076).

(Schmitt et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Lpin

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

some die during P-stage | heat sensitive

(Schmitt et al., 2015)

Phenotype Manifest In

lipid droplet | larval stage | heat sensitive

(Schmitt et al., 2015)

Detailed Description

Statement

Reference

Expression of Lpin^dsRNA.hs (induced by heat shock) does not result in any apparent larval developmental defect, but fat bodies in these larvae exhibit reduced lipid droplet size, as compared to controls, and pupae exhibit increased lethality, as compared to controls.

(Schmitt et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Lpin^RNAi.hs has some die during P-stage | heat sensitive phenotype, enhanceable by Tor[+]/mTor^k17004

(Schmitt et al., 2015)

Other

Statement

Reference

Lpin^RNAi.hs, Tor[+]/mTor^k17004 has visible | larval stage | heat sensitive phenotype

(Schmitt et al., 2015)

Lpin^RNAi.hs, Tor[+]/mTor^k17004 has abnormal size | larval stage | heat sensitive phenotype

(Schmitt et al., 2015)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Lpin^RNAi.hs has lipid droplet | larval stage | heat sensitive phenotype, non-enhanceable by Tor[+]/mTor^k17004

(Schmitt et al., 2015)

Other

Statement

Reference

Lpin^RNAi.hs, Tor[+]/mTor^k17004 has embryonic/larval fat body | larval stage | heat sensitive phenotype

(Schmitt et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Tor^k17004/+ in larvae expressing Lpin^dsRNA.hs (induced by heat shock) leads to a reduction in fat body mass and a transparent appearance of larvae; an increase in pupal lethality as compared to larvae expressing Lpin^dsRNA.hs in a wild type background; and the fat bodies from these larvae are smaller; but lipid droplets in the fat bodies of these larvae exhibit a similar size reduction as those in larvae expressing Lpin^dsRNA.hs in a wild type background.

(Schmitt et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lpin^RNAi.hs

Lpin^dsRNA.hs

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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