FlyBase Allele Report: Dmel\ohgt[Ex2]

General Information

Symbol

Dmel\ohgt^Ex2

Species

D. melanogaster

Name

FlyBase ID

FBal0324699

Feature type

allele

Associated gene

Dmel\ohgt

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

CRISPR/Cas9

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Wakabayashi et al., 2016)

Mutagen

CRISPR/Cas9

(Wakabayashi et al., 2016)

Progenitor genotype

Cytology

Description

Two nucleotide deletion within exon 2, producing a frameshift which is predicted to result in a truncated protein that contains only 15% of the full-length ohgt-PA protein (84 amino acid residues from the N terminus plus 7 amino acid residues, RGQSSQQ).

(Wakabayashi et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:10,049,619..10,049,620 [+]

Comment:

A 2 bp deletion leads to a frameshift in the second coding exon and early translation termination shortly thereafter.

(Wakabayashi et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | adult stage

(Wakabayashi et al., 2016)

abnormal body size | adult stage (with Df(3R)Exel6155)

(Wakabayashi et al., 2016)

abnormal body size | pupal stage

(Wakabayashi et al., 2016)

abnormal body size | pupal stage (with Df(3R)Exel6155)

(Wakabayashi et al., 2016)

abnormal developmental rate | larval stage

(Wakabayashi et al., 2016)

abnormal developmental rate | larval stage (with Df(3R)Exel6155)

(Wakabayashi et al., 2016)

abnormal size | adult stage

(Wakabayashi et al., 2016)

abnormal size | adult stage (with Df(3R)Exel6155)

(Wakabayashi et al., 2016)

fertile

(Wakabayashi et al., 2016)

increased cell number | adult stage

(Wakabayashi et al., 2016)

increased cell number | adult stage (with Df(3R)Exel6155)

(Wakabayashi et al., 2016)

viable

(Wakabayashi et al., 2016)

visible | larval stage

(Wakabayashi et al., 2016)

Phenotype Manifest In

wing blade

(Wakabayashi et al., 2016)

wing blade (with Df(3R)Exel6155)

(Wakabayashi et al., 2016)

Detailed Description

Statement

Reference

ohgt^Ex2 homozygotes are viable and fertile but about 30% display about 12 hr delay to pupate.

Despite a similar food intake to wild-type, ohgt^Ex2 homozygotes and ohgt^Ex2/Df(3R)Exel6155 have bigger body and organ size compared to controls, as assessed by body length in pupae and wet weight and wing area in adults - this is associated with increased cell number (measured in the posterior wing compartment).

(Wakabayashi et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ohgt^Ex2

(Wakabayashi et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close