FlyBase Allele Report: Dmel\Gba1a[KO]

General Information

Symbol

Dmel\Gba1a^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0325646

Feature type

allele

Associated gene

Dmel\Gba1a

Associated Insertion(s)

TI{TI}Gba1a^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Kinghorn et al., 2016)

Mutagen

ends-out gene targeting

(Kinghorn et al., 2016)

Progenitor genotype

Associated Insertion(s)

TI{TI}Gba1a^KO

Cytology

Description

The entire Gba1a open reading frame has been replaced by a loxP cassette that contains a w^+mW.hs marker.

(Kinghorn et al., 2016)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:23,700,639..23,702,545 [+]

Comment:

The Gba1a open reading frame has been replaced by a loxP cassette that contains a w^+mW.hs marker. Position of deletion endpoints on reference sequence determined from reported primer sequences used to generate homology arms.

(Kinghorn et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

long lived

(Kinghorn et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Gba1a^KO homozygous adults display significantly longer adult lifespan and do not show any defects in climbing ability across ages compared to controls.

(Kinghorn et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Gba1a^KO/Gba1a^KO is an enhancer of abnormal starvation stress response | adult stage phenotype of Gba1b^KO

(Kinghorn et al., 2016)

NOT Enhancer of

Statement

Reference

Gba1a^KO/Gba1a^KO is a non-enhancer of decreased fecundity | recessive | female | progressive phenotype of Gba1b^KO

(Kinghorn et al., 2016)

Gba1a^KO/Gba1a^KO is a non-enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Gba1b^KO

(Kinghorn et al., 2016)

Gba1a^KO/Gba1a^KO is a non-enhancer of abnormal oxidative stress response | adult stage phenotype of Gba1b^KO

(Kinghorn et al., 2016)

NOT Suppressor of

Statement

Reference

Gba1a^KO/Gba1a^KO is a non-suppressor of short lived phenotype of Gba1b^KO

(Kinghorn et al., 2016)

Gba1a^KO/Gba1a^KO is a non-suppressor of abnormal locomotor behavior | adult stage | progressive phenotype of Gba1b^KO

(Kinghorn et al., 2016)

Gba1a^KO/Gba1a^KO is a non-suppressor of abnormal oxidative stress response | adult stage phenotype of Gba1b^KO

(Kinghorn et al., 2016)

Gba1a^KO/Gba1a^KO is a non-suppressor of decreased fecundity | recessive | female | progressive phenotype of Gba1b^KO

(Kinghorn et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced adult lifespan along with the age-progressive decline in locomotor ability and decreased female fecundity characteristic for Gba1b^KO homozygous mutants is not modified by combination with Gba1a^KO in homozygous state.

The decreased survival rate upon starvation observed in Gba1b^KO homozygous adults is lowered further in Gba1b^KO,Gba1a^KO double mutants but the sensitivity of Gba1b^KO flies to oxidative stress is not worsened in the double mutants.

Gba1b^KO,Gba1a^KO double heterozygous flies have survival rate comparable to that of controls.

(Kinghorn et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Gba1a^KO

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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