FlyBase Allele Report: Dmel\wg[TOE.GS00125]

General Information

Symbol

Dmel\wg^TOE.GS00125

Species

D. melanogaster

Name

FlyBase ID

FBal0325737

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{TOE.GS00125}

Key Links

Transgenic product class

sgRNA

(Transgenic RNAi Project members, 2017-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

sgRNA

(Transgenic RNAi Project members, 2017-)

Mutagen

in vitro construct

(Transgenic RNAi Project members, 2017-)

Progenitor genotype

Carried in construct

P{TOE.GS00125}

Cytology

Description

CRISPR construct designed to result in activation of a target gene when combined with a source of nuclease-dead Cas9 fused to a transcriptional activator domain. Constitutively expresses two sgRNAs, both of which target sequence upstream of the transcription start site of wg.

(Transgenic RNAi Project members, 2017-)

Allele components

Component

Use(s)

Encoded product / tool

sgRNA-GS00125.1

sgRNA-GS00125.2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant | larval stage, with Scer\GAL4^en-e16E, Spyo\Cas9^{dCas9::VPR.UAS.Tag:FLAG}

(Ewen-Campen and Perrimon, 2024)

decreased cell death | larval stage | conditional, with Scer\GAL4^en-e16E, Spyo\Cas9^{dCas9::VPR.UAS.Tag:FLAG}

(Ewen-Campen and Perrimon, 2024)

radiation resistant | larval stage, with Scer\GAL4^en-e16E, Spyo\Cas9^{dCas9::VPR.UAS.Tag:FLAG}

(Ewen-Campen and Perrimon, 2024)

Phenotype Manifest In

wing disc | third instar larval stage, with Scer\GAL4^dpp.PU, Spyo\Cas9^{dCas9::VPR.UAS.Tag:FLAG}

(Lin et al., 2015)

wing disc posterior compartment | larval stage | conditional, with Scer\GAL4^en-e16E, Spyo\Cas9^{dCas9::VPR.UAS.Tag:FLAG}

(Ewen-Campen and Perrimon, 2024)

wing pouch | ectopic | third instar larval stage, with Scer\GAL4^dpp.PU, Spyo\Cas9^{dCas9::VPR.UAS.Tag:FLAG}

(Lin et al., 2015)

Detailed Description

Statement

Reference

Ectopic activation of the wg gene expression from its endogenous genomic locus using wg^TOE.GS00125 with Spyo\Cas9^{d.Scer\UAS.T:Zzzz\FLAG,T:Uuuu\nls-Unk,T:Zzzz\VPR} expressed under the control of Scer\GAL4^dpp.PU leads to partial duplication of the wing pouch in third instar larval wing discs.

(Lin et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

67545

y¹ sc^* v¹ sev²¹; P{TOE.GS00125}attP40

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

wg^TOE.GS00125

wg^sgRNA

Name Synonyms

Secondary FlyBase IDs

FBal0325385
FBal0322769

References (6)

Report Sections

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