FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Pink1S346D.KD.GMR.Tag:Mito(Tom20),GFP
Open Close
General Information
Symbol
Dmel\Pink1S346D.KD.GMR.Tag:Mito(Tom20),GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0325743
Feature type
allele
Associated gene
Dmel\Pink1
Associated Insertion(s)
Carried in Construct
M{GMR-Pink1.S346D.KD.Tom20.GFP}
Key Links
Transgenic product class
inframe_deletion
(Zhuang et al., 2016)
missense_variant
(Zhuang et al., 2016)
polypeptide_post_translational_processing_variant
(Zhuang et al., 2016)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Zhuang et al., 2016)
missense_variant
(Zhuang et al., 2016)
polypeptide_post_translational_processing_variant
(Zhuang et al., 2016)
Mutagen
in vitro construct
(Zhuang et al., 2016)
Progenitor genotype
Carried in construct
M{GMR-Pink1.S346D.KD.Tom20.GFP}
Cytology
Description

GMR regulatory sequences drive expression of Pink1 carrying both the D479A mutation that results in a kinase-dead form and the phosphomimetic mutation S346D (mutated residue is a conserved autophosphorylation site which appears to be the sole autophosphorylation site in Pink1). In addition, the N-terminal 93 amino acids of Pink1 have been replaced with the Tag:Mito(Tom20) outer mitochondrial membrane anchor signal and the protein is tagged with GFP.

(Zhuang et al., 2016)
Allele components
Component
Use(s)
Regulatory region(s)
GMR
Encoded product / tool
Pink1
Tagged with
GFP
Tag:Mito(Tom20)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  Parkinson's disease
      modeled by Pink1B9
      (Zhuang et al., 2016)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of parkGMR.T:Zzzz\FLAG with Pink1S346D.KD.GMR.T:Mito-Tom20,T:Avic\GFP) has no effect on the eye appearance and the flies display no loss of photoreceptors as they age.

      (Zhuang et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Pink1S346D.KD.GMR.Tag:Mito(Tom20),GFP rescues Pink1B9

      (Zhuang et al., 2016)
      Comments

      The reduced climbing ability, abnormal swollen mitochondria, disorganized muscle fibres as well as the decreased number of brain dopaminergic neurons characteristic for Pink1B9 adults is rescued by combination with Pink1S346D.KD.GMR.T:Mito-Tom20,T:Avic\GFP.

      (Zhuang et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Pink1S346D.KD.GMR.T:Mito-Tom20,T:Avic\GFP
      Pink1S346D.KD.GMR.Tag:Mito(Tom20),GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)