Sequence composed of a linker followed by the mCherry open reading frame and a Tag:FLAG epitope have been inserted at the C-terminal end of the Pink1 coding sequence, resulting in the tagging of the endogenous Pink1 gene.
Pink1mCherry homozygous adults and Pink1mCherry/Pink1B9 transheterozygous adults display no obvious wing posture defects, as compared to Pink1B9 heterozygous controls.