FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Marcal1K275M
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General Information
Symbol
Dmel\Marcal1K275M
Species
D. melanogaster
Name
FlyBase ID
FBal0326309
Feature type
allele
Associated gene
Dmel\Marcal1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Holsclaw and Sekelsky, 2017)
Progenitor genotype
Cytology
Description

Amino acid replacement: K275M.

(Holsclaw and Sekelsky, 2017)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
2L:4,956,464..4,956,465 [+]
Nucleotide change:

AA4956465TG

Reported amino acid change:

K275M

Comment:

AAA to ATG mutation at K275 of Marcal1.

(Holsclaw and Sekelsky, 2017)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Marcal1K275M mutants show defects in the DNA double-strand break repair as assessed by the P{hswa} and P{wIw.FRT} male germline assay: the mutants display significantly reduced synthesis-dependent strand annealing (SDSA) repair (both in percentage of total progeny scored and in number of males with observable SDSA events in the progeny) and decreased end joining events as well as reduced single-strand annealing (SSA) capacity relative to wild-type controls.

      (Holsclaw and Sekelsky, 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Marcal1K275M
      (Holsclaw and Sekelsky, 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)