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General Information
Symbol
Scer\GAL4DBD.R72E01
Species
S. cerevisiae
Name
FlyBase ID
FBal0326456
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
R72E01_ZpGdbd
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The R72E01 enhancer fragment is fused upstream of the Drosophila synthetic core promoter (DSCP) followed by the GAL4(DBD)::Zip- hemidriver (component of the 'Split GAL4' system, contains the GAL4 DNA-binding domain). A Hsp70 transcriptional terminator is present.

Allele components
Product class / Tool use(s)
Associated Sequence Features
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
lobula columnar neuron Lcn4

Comment: when combined with P{R47H03-p65.AD} (combination referred to as 'SS00315')

adult ventral nervous system | restricted

Comment: when combined with P{R47H03-p65.AD} (combination referred to as 'SS00315')

Additional Information
Statement
Reference

In a split-GAL4 system, Scer\GAL4DBD.R72E01 drives expression in lobula columnar neuron Lcn4 and a subset of neurons in the thoracico-abdominal ganglion when combined with Hsap\RELAAD.R47H03.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R72E01 and Hsap\RELAAD.R47H03 in adults reduces the depolarisation of the giant fiber neuron in response to fast angular velocity stimuli, compared to controls.

Optogenetic activation (using Cnoc\ChR1Cs.IVS.20xUAS.Venus with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R72E01 and Hsap\RELAAD.R47H03 in adults evokes a short latency transient depolarisation, followed by a second long-lasting depolarization, of the giant fiber neuron, not seen in controls, consisting of broad depolarizations and narrow spikes; activation of these cells, or neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R72E01 and Hsap\RELAAD.R49C04, significantly increases takeoff rates, compared to controls; takeoffs are shorter in duration than spontaneous takeoffs in controls.

Silencing (using Hsap\KCNJ2IVS.10xUAS.EGFP) of neurons targeted by the split-GAL4 pair of Scer\GAL4DBD.R72E01 and Hsap\RELAAD.R47H03, or the split-GAL4 pair of Scer\GAL4DBD.R72E01 and Hsap\RELAAD.R49C04, in adults significantly reduces overall takeoff rates and the proportion of short takeoffs in response to fast, but not slow, looming stimuli, compared to controls; in the case of Scer\GAL4DBD.R72E01 and Hsap\RELAAD.R47H03, there is also significantly reduced activity of the giant fiber neuron at the appearance of objects and the approach of fast, but not slow, looming stimuli, with no effect on hyperpolarization when objects stop moving, compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Split-GAL4 transgene, constructed as described in FBrf0212052.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Scer\GAL4DBD.R72E01.T:Zzzz\Zip-
Scer\GAL4DBD.R72E01
Name Synonyms
Secondary FlyBase IDs
    References (5)