The inactivation of lobula columnar neurons LC4 (by the expression of Ctet\tetX^TNT-LC.UAS under the control of the split-Gal4 Scer\GAL4^DBD.R72E01+Hsap\RELA^AD.R47H03) leads to adults failing to decrease their copulation latency upon exposure to the parasitic wasps Leptopilina heterotoma, Leptopilina boulardi, Trichopria drosophilae and Asobara tabida.

Inhibition (using Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R72E01 and Hsap\RELA^AD.R47H03 in adults reduces the depolarisation of the giant fiber neuron in response to fast angular velocity stimuli, compared to controls.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 590 nm (red) light) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R72E01 and Hsap\RELA^AD.R47H03 in adults evokes a short latency transient depolarisation, followed by a second long-lasting depolarization, of the giant fiber neuron, not seen in controls, consisting of broad depolarizations and narrow spikes; activation of these cells, or neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R72E01 and Hsap\RELA^AD.R49C04, significantly increases takeoff rates, compared to controls; takeoffs are shorter in duration than spontaneous takeoffs in controls.

Silencing (using Hsap\KCNJ2^{IVS.10xUAS.EGFP}) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R72E01 and Hsap\RELA^AD.R47H03, or the split-GAL4 pair of Scer\GAL4^DBD.R72E01 and Hsap\RELA^AD.R49C04, in adults significantly reduces overall takeoff rates and the proportion of short takeoffs in response to fast, but not slow, looming stimuli, compared to controls; in the case of Scer\GAL4^DBD.R72E01 and Hsap\RELA^AD.R47H03, there is also significantly reduced activity of the giant fiber neuron at the appearance of objects and the approach of fast, but not slow, looming stimuli, with no effect on hyperpolarization when objects stop moving, compared to controls.