FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rgk1RNAi.UAS
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General Information
Symbol
Dmel\Rgk1RNAi.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0326529
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

UAS regulatory sequences drive expression of an artificial microRNA (shRNA) targeting Rgk1.

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of Rgk1dsRNA.shRNA.Scer\UAS under the control of Scer\GAL4ey-OK107, Scer\GAL42765, Scer\GAL4c739, Scer\GAL4Rgk1-NP5225 (an intrinsic Gal4 driver of Rgk1), or Scer\GAL4Mef2.247, but not Scer\GAL41993, leads to a significant decrease in intermediate-term olfactory memory (2h after conditioning) in adults, assessed by olfactory aversive conditioning assays, as compared to controls; expression under the control of Scer\GAL4ey-OK107 alone also leads to a significant decrease in short-term olfactory memory (5min after conditioning) in adults, as compared to controls; expression under the combined control of Scer\GAL4ey-OK107 and Gal80[ts] in order to only transiently express for 3 days during adulthood leads to an equally transient decrease in intermediate-term memory, as compared to controls. None of the above expression conditions results in significant defects in olfactory acuity, response to electric shocks, or morphology of the mushroom body, as compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Rgk1RNAi.UAS
Rgk1dsRNA.shRNA.Scer\UAS
Rgk1dsRNA.shRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)