FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\T48lexAop.UAS.GrabFP.A.Ext.mCherry
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General Information
Symbol
Dmel\T48lexAop.UAS.GrabFP.A.Ext.mCherry
Species
D. melanogaster
Name
FlyBase ID
FBal0326547
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Carried in construct
Cytology
Description

lexAop and UAS regulatory sequences and a minimal Hsp70 promoter (derived from the pUASTLOTattB vector) are fused upstream of a fusion protein that encodes an apically enriched nanobody-based GFP trap for extracellular proteins. The fusion protein contains the T48 coding sequence with the GFP-binding VHH(GFP4) nanobody inserted near the N-terminal end. In addition, a mCherry fluorescent tag followed by the minimal apical localization sequence of baz are present at the C-terminal end of the T48 coding sequence. The VHH(GFP4) nanobody faces the extracellular space. The lexAop and UAS regulatory sequences are flanked by loxP and lox2272 sites respectively (meaning that the P1cre recombinase can be used to excise one of the enhancers in a mutually exclusive manner if desired).

Allele components
Component
Use(s)
Encoded product / tool
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DNA sequence
Protein sequence
 
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
T48Ecol\lexAop.Scer\UAS.GrabFP.A.Ext.T:Disc\RFP-mCherry
T48lexAop.UAS.GrabFP.A.Ext.mCherry
Name Synonyms
Secondary FlyBase IDs
    References (1)