UASp regulatory sequences drive expression of full-length Khc, tagged with TagBFP2.
Scer\GAL4VP16.nos.UTR/KhcUASp.TagBFP2 rescues KhcKO.mutA
KhcUASp.TagBFP2/Scer\GAL4elav.PLu partially rescues KhcKO.mutA
KhcUASp.TagBFP2/Scer\GAL4elav.PLu partially rescues Khc27
The reduced velocity of ooplasmic streaming characteristic for KhcKO.mutA mutant oocytes is fully rescued by expression of KhcScer\UAS.P\T.T:Equa\eqFP578-TagBFP2 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the mutant background.
The lethality of Khc27 mutant larvae is strongly delayed by Scer\GAL4elav.PLu-driven expression of KhcScer\UAS.P\T.T:Equa\eqFP578-TagBFP2, but all the Khc27 mutants die before pupariation regardless of the expression the transgene.