FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\TpiG75E
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General Information
Symbol
Dmel\TpiG75E
Species
D. melanogaster
Name
FlyBase ID
FBal0327046
Feature type
allele
Associated gene
Dmel\Tpi
Associated Insertion(s)
TI{TI}TpiG75E
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Roland et al., 2016)
Progenitor genotype
TpiattP
(Roland et al., 2016)
Associated Insertion(s)
TI{TI}TpiG75E
Cytology
Description

A mutated copy of the Tpi locus (containing the amino acid replacement G74E) has been inserted into the attP site present in TpiattP. This mutation is predicted to result in disruption of dimerization of the Tpi protein.

(Roland et al., 2016)

(FlyBase curator comment: the mutation in the Tpi gene is given as G74E in FBrf0233082, however analysis of the release 6.40 annotated gene model indicates the change to be G75E).

(Roland et al., 2016)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
MNV
3R:30,133,822..30,133,823 [-]
Reported amino acid change:

G74E

Comment:

A mutated copy of the Tpi locus (containing the amino acid replacement G74E) has been inserted into the attP site present in TpiattP. A two base substitution is required for this G to E codon change.

(Roland et al., 2016)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      TpiG74E homozygotes are partially lethal; TpiG74E/TpiK11M transheterozygous and TpiG74E homozygous adults, but not TpiG74E heterozygous adults, exhibit an increase in heat sensitivity, shown by the significant decrease in the time until paralysis after a temperature shift to 38[o]C, and an increase in bang sensitivity, shown by a significant increase in recovery time from mechanical stress, as compared to controls; TpiG74E/TpiK11M transheterozygous and TpiG74E homozygous adults also exhibit a significant decrease in lifespan, whereas TpiG74E heterozygous adults exhibit a significant increase in lifespan, as compared to controls.

      TpiG74E/TpiG74E-CFP transheterozygotes exhibit an increase in heat sensitivity and an increase in bang sensitivity during adulthood, while showing insignificant changes in the numbers of boutons and branches at the third instar larval neuromuscular junction, as compared to controls.

      (Roland et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TpiG74E
      TpiG75E
      dTPIG74E
      (Roland et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)