wstd, sugarkill, ND14
Gene model reviewed during 5.47
1.3 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tpi using the Feature Mapper tool.
Tpi transcripts are detected throughout development on northern blots. They are very abundant in very early embryos and then decline. Levels then rise to a peak in third instar larvae followed by a decline and a subsequent rise near the time of eclosion. This is a pattern characteristic of enzymes involved in intermediate metabolism.
Glycolytic enzymes, including Tpi protein are localized to M bands and Z discs, as visualized in the isolated myofibrils of flight muscles, where immunoreactivity appears as alternating bright and less-intense bands.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Tpi in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Mutants have reduced longevity, progressive locomotor deficiency and neural degeneration.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Identified in 2D gels of CMW W2 wing imaginal disc cell proteins.
Tpi has been cloned and sequenced, and its developmental expression pattern analysed.
Structural gene for the glycolytic enzyme triosephosphate isomerase (TPI) found in muscle. Heterozygote contains the parental isozymes plus a hybrid isozyme.