FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\TpiK11M
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General Information
Symbol
Dmel\TpiK11M
Species
D. melanogaster
Name
FlyBase ID
FBal0293726
Feature type
allele
Associated gene
Dmel\Tpi
Associated Insertion(s)
TI{TI}TpiK11M
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Roland et al., 2013)
cre recombinase
(Roland et al., 2013)
Progenitor genotype
TpiattP
(Roland et al., 2013)
Associated Insertion(s)
TI{TI}TpiK11M
Cytology
Description

A mutated copy of the Tpi locus (containing the amino acid replacement K11M, resulting in a catalytically inactive protein) has been inserted into the attP site present in TpiattP. A single attR site remains 5' and a single loxP site remains 3' to the reconstituted Tpi locus.

(Roland et al., 2013)
Allele components
Component
Use(s)
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  triosephosphate isomerase deficiency
      modeled by Tpisgk-1
      (Roland et al., 2013)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      TpiK11M/TpiT73R, TpiK11M/TpiG74E and TpiK11M/TpiT73R.G74E exhibit an increase in heat sensitivity, shown by the significant decrease in the time until paralysis after a temperature shift to 38[o]C, as compared to controls. TpiK11M/TpiG74E and TpiK11M/TpiT73R.G74E transheterozygous adults exhibit a significant decrease in lifespan, whereas TpiK11M/TpiT73R transheterozygous adults exhibit a significant increase in lifespan, as compared to controls. TpiG74E/TpiK11M transheterozygous adults, but not TpiK11M/TpiT73R and TpiT73R.G74E/TpiK11M transheterozygous adults, exhibit an increase in bang sensitivity, shown by a significant increase in recovery time from mechanical stress, as compared to controls.

      TpiK11M/TpiT73R transheterozygous third instar larval neuromuscular junction neurons also exhibit a significant decrease in endocytosis at 38[o]C temperature conditions, as compared to controls.

      (Roland et al., 2016)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially complements

      Tpisgk-1

      (Roland et al., 2013)
      Comments

      TpiK11M fully complements the mechanical and thermal stress sensitive phenotypes of Tpisgk-1, at both 3 days and 20 days after eclosion. TpiK11M also complements the reduced longevity phenotype of Tpisgk-1. TpiK11M fails to complement the markedly reduced isomerase activity seen in Tpisgk-1.

      (Roland et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      TPIK11M
      (Roland et al., 2013)
      TPIK11M (attR)
      (Roland et al., 2013)
      TpiK11M
      dTPIΔcat
      (Roland et al., 2016)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)