FlyBase Allele Report: Dmel\Tpi[T74R.G75E]

General Information

Symbol

Dmel\Tpi^T74R.G75E

Species

D. melanogaster

Name

FlyBase ID

FBal0327047

Feature type

allele

Associated gene

Dmel\Tpi

Associated Insertion(s)

TI{TI}Tpi^T74R.G75E

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

phiC31 integrase

Nature of the Allele

Allele class

Mutagen

phiC31 integrase

(Roland et al., 2016)

Progenitor genotype

Tpi^attP

(Roland et al., 2016)

Associated Insertion(s)

TI{TI}Tpi^T74R.G75E

Cytology

Description

A mutated copy of the Tpi locus (containing the amino acid replacements T73R and G74E) has been inserted into the attP site present in Tpi^attP. These mutations are predicted to result in disruption of dimerization of the Tpi protein.

(Roland et al., 2016)

(FlyBase curator comment: the mutations in the Tpi gene is given as T73R and G74E in FBrf0233082, however analysis of the release 6.40 annotated gene model indicates the changes to be T74R and G75E respectively).

(Roland et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

MNV

3R:30,133,825..30,133,826 [-]

Comment:

A mutated copy of the Tpi locus (containing the amino acid replacements T74R and G75E) has been inserted into the attP site present in Tpi^attP. This is the site of the first of the two codon changes leading to T74R amino acid change. Note that the change is indicated as T73R in the paper.

(Roland et al., 2016)

MNV

3R:30,133,822..30,133,823 [-]

Comment:

A mutated copy of the Tpi locus (containing the amino acid replacements T74R and G75E) has been inserted into the attP site present in Tpi^attP. This is the site of the second of the two codon changes leading to the G75E amino acid change. Note that the change is indicated as G74E in the paper.

(Roland et al., 2016)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of triosephosphate isomerase deficiency

CEA

(Roland et al., 2016)

CEA with Tpi^K11M

(Roland et al., 2016)

CEA with Tpi^{T73R.G74E-CFP}

(Roland et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response | adult stage (with Tpi^K11M)

(Roland et al., 2016)

abnormal heat stress response | adult stage (with Tpi^{T73R.G74E-CFP})

(Roland et al., 2016)

abnormal heat stress response | adult stage | recessive

(Roland et al., 2016)

bang sensitive | adult stage (with Tpi^{T73R.G74E-CFP})

(Roland et al., 2016)

bang sensitive | adult stage | recessive

(Roland et al., 2016)

long lived | dominant

(Roland et al., 2016)

partially lethal

(Roland et al., 2016)

short lived (with Tpi^K11M)

(Roland et al., 2016)

short lived | recessive

(Roland et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Tpi^T73R.G74E/Tpi^K11M transheterozygous and Tpi^T73R.G74E homozygous adults, but not Tpi^T73R.G74E heterozygous adults, exhibit an increase in heat sensitivity, shown by the significant decrease in the time until paralysis after a temperature shift to 38[o]C, as compared to controls; Tpi^T73R.G74E homozygous adults, but not Tpi^T73R.G74E/Tpi^K11M transheterozygous and Tpi^T73R.G74E heterozygous adults, exhibit an increase in bang sensitivity, shown by a significant increase in recovery time from mechanical stress, as compared to controls; Tpi^T73R.G74E/Tpi^K11M transheterozygous also exhibit a significant decrease in lifespan, whereas Tpi^T73R.G74E heterozygous adults exhibit a significant increase in lifespan, as compared to controls.

Tpi^T73R.G74E/Tpi^{T73R.G74E-CFP} transheterozygous adults exhibit an increase in heat sensitivity and an increase in bang sensitivity during adulthood, as compared to controls.

(Roland et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tpi^T73R.G74E

Tpi^T74R.G75E

dTPI^T73R,G74E

(Roland et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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