Tpi^T73R homozygotes are partially lethal; Tpi^T73R homozygous, but not heterozygous, adults exhibit an increase in heat sensitivity, shown by the significant decrease in the time until paralysis after a temperature shift to 38[o]C, and an increase in bang sensitivity, shown by a significant increase in recovery time from mechanical stress, as compared to controls; Tpi^T73R homozygous adults exhibit a significant decrease in lifespan, whereas their heterozygous counterparts exhibit a significant increase in lifespan, as compared to controls. Tpi^T73R homozygous third instar larval neuromuscular junction neurons exhibit a significant and progressive decrease in endocytosis at 38[o]C temperature conditions, but not at room temperature, while exhibiting insignificant changes in exocytosis upon stimulation by high potassium concentration at 38[o]C temperature conditions, as compared to controls;

Tpi^T73R/Tpi^K11M transheterozygous adults exhibit a significant increase in lifespan, a small but significant increase in heat sensitivity, but insignificant changes in bang sensitivity, as compared to controls. Their third instar larval neuromuscular junction neurons also exhibit a significant decrease in endocytosis at 38[o]C temperature conditions, as compared to controls.

Tpi^T73R/Tpi^T73R-CFP transheterozygotes exhibit an increase in heat sensitivity and an increase in bang sensitivity during adulthood, and their third instar larval neuromuscular junction neurons show insignificant changes in the numbers of boutons and branches, as compared to controls.