FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\TpihTPI.WT
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General Information
Symbol
Dmel\TpihTPI.WT
Species
D. melanogaster
Name
FlyBase ID
FBal0320061
Feature type
allele
Associated gene
Dmel\Tpi
Associated Insertion(s)
TI{TI}TpihTPI.WT
Carried in Construct
Key Links
Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Roland et al., 2015)
Mutagen
phiC31 integrase
(Roland et al., 2015)
cre recombinase
(Roland et al., 2015)
Progenitor genotype
Associated Insertion(s)
TI{TI}TpihTPI.WT
Cytology
Description

The endogenous Tpi coding region has been removed. Sequence encoding wild-type Hsap\TPI1 has been inserted in its place by phiC31:int-mediated integration into the attP site present in TpiattP.

(Roland et al., 2015)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      DOES NOT model  triosephosphate isomerase deficiency
      CEA with Tpisgk-js10, Hsap\TPI1Tpi.WT
      (Roland et al., 2016)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Tpisgk-js10/TpihTPI.WT heterozygotes show a low-penetrant change in thermal stress sensitivity, compared to TpihTPI.WT/TpihTPI.WT flies. Compared to Tpisgk-js10/TpihTPI.WT control heterozygotes, Tpisgk-js10/TpihTPI.I170V flies (both 3-4 and 20-22 days old) are more sensitive to mechanical- (significant increase in recovery time) and thermal- (significant decrease in time to paralysis) stress, but do not show significant changes in lifespan.

      (Roland et al., 2015)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of Hsap\TPI1Tpi.WT (in which the coding sequence of endogenous Tpi is replaced by the human ortologue sequence) in an effectively Tpi-null background (i.e. TpihTPI.WT/Tpisgk-js10) leads to adult exhibiting a small increase in lifespan, but to no obvious defects in bang sensitivity or heat sensitivity, as compared to controls.

      (Roland et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      TpihTPI.WT
      Name Synonyms
      Secondary FlyBase IDs
        References (2)