- Tools
- Downloads
- Links
- Community
- About
- Help
FB2026_01
,
released March 12, 2026
1.6 kb deletion resulting from the imprecise excision of the P{EPgy2}TpiEY03361 element. The deletion extends from -319 to +1288 relative to the start of exon 1.
bang sensitive (with TpiM80T)
bang sensitive | adult stage (with TpiM80T)
short lived (with TpiM80T)
short lived (with Tpisgk-1)
TpiR187K/Tpisgk-js10 and TpiM80T/Tpisgk-js10 individuals are bang sensitive.
TpiM80T/Tpisgk-js10 transheterozygous adults exhibit a significant decrease in lifespan, an increase in heat sensitivity, shown by the significant decrease in the time until paralysis after 38[o]C temperature shift, and an increase in bang sensitivity, shown by a significant increase in recovery time from mechanical stress, as compared to controls.
The presence of one copy of Hsap\TPI1M82T.Tpi (in which the coding sequence of endogenous Tpi is replaced by the human ortologue bearing the M82T amino-acid substitution), but not of one copy of Hsap\TPI1Tpi.WT (in which the coding sequence of endogenous Tpi is replaced by the human ortologue bearing the M82T amino-acid substitution), in a Tpi-null background (TpihTPI.M82T/Tpisgk-js10 or TpihTPI.WT/Tpisgk-js10, respectively) leads to adult exhibiting a small decrease in lifespan, a small increase in bang sensitivity, shown by a small but significant increase in recovery time from mechanical stress, and an increase in heat sensitivity, shown by the significant decrease in the time until paralysis after a temperature shift to 38[o]C, as compared to controls.
Compared to Tpisgk-js10/TpihTPI.WT control heterozygotes, Tpisgk-js10/TpihTPI.I170V flies (both 3-4 and 20-22 days old) are more sensitive to mechanical- (significant increase in recovery time) and thermal- (significant decrease in time to paralysis) stress, but do not show significant changes in lifespan.
Ratios of redox molecules are significantly shifted towards the oxidised form in Tpisgk-1/Tpisgk-js10 animals compared to controls. This phenotype is age-dependent.
Tpisgk-1 homozygotes and Tpisgk-1/Tpisgk-js10 flies show a delay in recovery after mechanical stress compared to controls, at both 3 and 20 days of age.
Tpisgk-1 homozygotes and Tpisgk-1/Tpisgk-js10 flies show paralysis upon thermal stress, at both 3 and 20 days of age.
Tpisgk-1 homozygotes and Tpisgk-1/Tpisgk-js10 flies show significantly reduced median longevity compared to controls.
Homozygotes do not survive beyond larval stage 2.
Tpisgk-1/Tpisgk-js10 flies take longer to regain normal locomotion after mechanical stress than control flies. This stress sensitivity is progressive and increases significantly with the age of the flies at all temperatures examined (room temperature, 25oC and 29oC). Severe stress sensitivity is evident much earlier in flies maintained at 25oC or 29oC compared to those raised at room temperature.
Lifespan of homozygous and Tpisgk-1/Tpisgk-js10 animals is significantly reduced compared to controls at room temperature, 25oC and 29oC. The decrease in lifespan compared to controls becomes more severe as the temperature at which the flies are raised is increased.
Young Tpisgk-1/Tpisgk-js10 adults do not show neuropathological defects, however, by their median age, they develop marked neuropathology, showing degeneration in the brain and thoracic ganglion. This degeneration is seen in flies raised at room temperature, 25oC and 29oC.
Hsap\TPI1M82T.Tpi, Tpisgk-js10/TpihTPI.M82T has short lived phenotype
Hsap\TPI1M82T.Tpi, Tpisgk-js10/TpihTPI.M82T has abnormal heat stress response | adult stage phenotype
Hsap\TPI1M82T.Tpi, Tpisgk-js10/TpihTPI.M82T has bang sensitive | adult stage phenotype
Hsap\TPI1Tpi.WT, Tpisgk-js10/TpihTPI.M82T has long lived phenotype