FlyBase Allele Report: Dmel\Tpi[GD10138]

General Information

Symbol

Dmel\Tpi^GD10138

Species

D. melanogaster

Name

FlyBase ID

FBal0208826

Feature type

allele

Associated gene

Dmel\Tpi

Associated Insertion(s)

Carried in Construct

P{GD10138}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10138}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10138

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

DOES NOT model triosephosphate isomerase deficiency

CEA

(Roland et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Vicente Miranda et al., 2017)

exacerbates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Vicente Miranda et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic myoblast, with Scer\GAL4^kirre.PU

(Tixier et al., 2013)

larval somatic muscle cell, with Scer\GAL4^kirre.PU

(Tixier et al., 2013)

Detailed Description

Statement

Reference

The expression of Tpi^GD10138 under the control of Scer\GAL4^elav-C155 leads to a significant and progressive decrease in adult locomotor capacity in negative geotaxis assays, and leads to a small but significant decrease in lifespan, as compared to controls.

(Vicente Miranda et al., 2017)

Adult expressing Tpi^GD10138 under the control of Scer\GAL4^Act5C.PI exhibit hypoactivity, but do not exhibit any obvious increase in bang sensitivity or heat sensitivity, as compared to controls.

(Roland et al., 2016)

Embryos expressing Tpi^GD10138 under the control of Scer\GAL4^kirre.PU have thinner muscles than normal and unfused myoblasts are present.

(Tixier et al., 2013)

Adults expressing Tpi^GD10138 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10138

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav-C155, Tpi^GD10138 has abnormal locomotor behavior | adult stage | progressive phenotype, enhanceable by Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Vicente Miranda et al., 2017)

Scer\GAL4^elav-C155, Tpi^GD10138 has short lived phenotype, enhanceable by Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Vicente Miranda et al., 2017)

Enhancer of

Statement

Reference

Tpi^GD10138, Scer\GAL4^elav-C155 is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Vicente Miranda et al., 2017)

Tpi^GD10138, Scer\GAL4^elav-C155 is an enhancer of short lived phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^elav-C155

(Vicente Miranda et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of Tpi^GD10138 and Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^elav-C155 leads to a significant and progressive decrease in adult locomotor capacity (in negative geotaxis assays) and a significant decrease in lifespan; former is more severe than either single expression conditions, whereas the later is only more severe than the Tpi^GD10138 single expression condition.

(Vicente Miranda et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v25643

w¹¹¹⁸; P{GD10138}v25643

v25644

w¹¹¹⁸; P{GD10138}v25644