FB2026_02 , released June 18, 2026
Allele: Dmel\Nep1mut.UAS
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General Information
Symbol
Dmel\Nep1mut.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0336290
Feature type
allele
Associated gene
Dmel\Nep1
Associated Insertion(s)
Carried in Construct
P{UAS-Nep1.mut}
Key Links
Transgenic product class
missense_variant
(Turrel et al., 2017)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Turrel et al., 2017)
Mutagen
in vitro construct
(Turrel et al., 2017)
Progenitor genotype
Carried in construct
P{UAS-Nep1.mut}
Cytology
Description

UASt regulatory sequences drive expression of Nep1 cDNA (DGRC GH03315) with a mutation that disrupts the catalytic activity of the enzyme (E685V).

(Turrel et al., 2017)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Nep1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Nep1mut.Scer\UAS under the control of Scer\GAL4VT064246 (using tub-Gal80[ts] to limit the expression to adulthood) does not impair long-term memory tested in an aversive olfactory memory assay 24hr post-training.

      (Turrel et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The impaired long-term memory tested in an aversive olfactory memory assay (measured 24hr post-training) characteristic for adult flies expressing ApplAβ.Scer\UAS.T:Ivir\HA1 (combined with tub-Gal80[ts] to limit expression to adulthood) under the control of Scer\GAL4VT064246 is not significantly affected by co-expression of Nep1mut.Scer\UAS.

      (Turrel et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Nep1mut.Scer\UAS
      Nep1mut.UAS
      Nep1mut
      (Turrel et al., 2017)
      Name Synonyms
      Secondary FlyBase IDs
        References (1)